{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ewoldt JK"],"funding":["NIDCR NIH HHS","NHLBI NIH HHS","National Science Foundation (NSF)","U.S. Department of Health & Human Services | National Institutes of Health (NIH)","U.S. Department of Health & Human Services | National Institutes of Health","National Science Foundation"],"pagination":["24-40"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12376733"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["Recent innovations in differentiating cardiomyocytes from human induced pluripotent stem cells (hiPSCs) have unlocked a viable path to creating in vitro cardiac models. Currently, hiPSC-derived cardiomyocytes (hiPSC-CMs) remain immature, leading many in the field to explore approaches to enhance cell and tissue maturation. Here, we systematically analyzed 300 studies using hiPSC-CM models to determine common fabrication, maturation and assessment techniques used to evaluate cardiomyocyte functionality and maturity and compiled the data into an open-access database. Based on this analysis, we present the diversity of, and current trends in, in vitro models and highlight the most common and promising practices for functional assessments. We further analyzed outputs spanning structural maturity, contractile function, electrophysiology and gene expression and note field-wide improvements over time. Finally, we discuss opportunities to collectively pursue the shared goal of hiPSC-CM model development, maturation and assessment that we believe are critical for engineering mature cardiac tissue."],"journal":["Nature methods"],"pubmed_title":["Induced pluripotent stem cell-derived cardiomyocyte in vitro models: benchmarking progress and ongoing challenges."],"pmcid":["PMC12376733"],"funding_grant_id":["EEC-1647837","2033654","F31 HL158195-03","GRFP","T32-HL125242","T32-DE007057","R01 HL147585","F31 HL158195","T32 HL125242","T32 DE007057"],"pubmed_authors":["Lejeune E","Ma M","Salazar Coariti AC","He J","Toussaint KC","Ramaswamy S","Lin YM","Mir Hashemian P","Ewoldt JK","Bifano TG","Agarwal A","McLellan MA","Baker BM","Karakan MC","White AE","Chen CS","Jewett ME","Gao X","Tabares J","DePalma SJ","Lou L"],"additional_accession":[]},"is_claimable":false,"name":"Induced pluripotent stem cell-derived cardiomyocyte in vitro models: benchmarking progress and ongoing challenges.","description":"Recent innovations in differentiating cardiomyocytes from human induced pluripotent stem cells (hiPSCs) have unlocked a viable path to creating in vitro cardiac models. Currently, hiPSC-derived cardiomyocytes (hiPSC-CMs) remain immature, leading many in the field to explore approaches to enhance cell and tissue maturation. Here, we systematically analyzed 300 studies using hiPSC-CM models to determine common fabrication, maturation and assessment techniques used to evaluate cardiomyocyte functionality and maturity and compiled the data into an open-access database. Based on this analysis, we present the diversity of, and current trends in, in vitro models and highlight the most common and promising practices for functional assessments. We further analyzed outputs spanning structural maturity, contractile function, electrophysiology and gene expression and note field-wide improvements over time. Finally, we discuss opportunities to collectively pursue the shared goal of hiPSC-CM model development, maturation and assessment that we believe are critical for engineering mature cardiac tissue.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-05-09T17:49:00.258Z","creation":"2026-04-08T01:08:20.647Z"},"accession":"S-EPMC12376733","cross_references":{"pubmed":["39516564"],"doi":["10.1038/s41592-024-02480-7"]}}