{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin X"],"funding":["Bill and Melinda Gates Foundation (GF)","NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["e2510163122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12377726"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(33)"],"pubmed_abstract":["The development of germline-targeting vaccines represents a potentially transformative strategy to elicit broadly neutralizing antibodies (bnAbs) against HIV and other antigenically diverse pathogens. Here, we report on structural characterization of vaccine-elicited VRC01-class bnAb precursors in the IAVI G001 Phase 1 clinical trial with the eOD-GT8 60mer nanoparticle as immunogen. High-resolution X-ray structures of eOD-GT8 monomer complexed with Fabs of five VRC01-class bnAb precursors with >90% germline identity revealed a conserved mode of binding to the HIV CD4-binding site via IGHV1-2-encoded heavy chains, mirroring mature bnAb interactions. The light-chain V-gene diversity emulated VRC01 bnAbs and stabilized antigen engagement, while their conserved five-residue LCDR3 motifs preven"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Structural insights into VRC01-class bnAb precursors with diverse light chains elicited in the IAVI G001 human vaccine trial."],"pmcid":["PMC12377726"],"funding_grant_id":["CAVD","P41 GM103393","UM1 AI144462"],"pubmed_authors":["Lin X","Lu D","Wilson IA","Yuan M","Kalyuzhniy O","Tingle R","Kubitz M","Schief WR","Cottrell CA"],"additional_accession":[]},"is_claimable":false,"name":"Structural insights into VRC01-class bnAb precursors with diverse light chains elicited in the IAVI G001 human vaccine trial.","description":"The development of germline-targeting vaccines represents a potentially transformative strategy to elicit broadly neutralizing antibodies (bnAbs) against HIV and other antigenically diverse pathogens. Here, we report on structural characterization of vaccine-elicited VRC01-class bnAb precursors in the IAVI G001 Phase 1 clinical trial with the eOD-GT8 60mer nanoparticle as immunogen. High-resolution X-ray structures of eOD-GT8 monomer complexed with Fabs of five VRC01-class bnAb precursors with >90% germline identity revealed a conserved mode of binding to the HIV CD4-binding site via IGHV1-2-encoded heavy chains, mirroring mature bnAb interactions. The light-chain V-gene diversity emulated VRC01 bnAbs and stabilized antigen engagement, while their conserved five-residue LCDR3 motifs preven","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-07-15T08:58:34.037Z","creation":"2026-07-03T03:10:42.764Z"},"accession":"S-EPMC12377726","cross_references":{"pubmed":["40789024"],"doi":["10.1073/pnas.2510163122"]}}