biostudies-literatureXue JScience and Technology Planning Project of Guizhou Province662https://www.ebi.ac.uk/biostudies/studies/S-EPMC12384973biostudies-literatureUnknown47(8)Aralia chinensis L. has shown potential in breast cancer treatment, yet its pharmacodynamically active components and mechanisms remain undefined. To systematically identify the bioactive constituents absorbed into the bloodstream and elucidate their multi-target mechanisms against breast cancer, we employed ultra-high-performance liquid chromatography in conjunction with Q Exactive Orbitrap mass spectrometry (UHPLC-Q Exactive Orbitrap-MS) alongside serum pharmacochemistry to analyze the chemical constituents of total saponins derived from A. chinensis (TSAC) and to identify the blood-absorbed prototypes in a rat model. Network pharmacology predicted targets and pathways of serum prototypes, validated by molecular docking and in vitro experiments. We identified 38 triterpenoid saponins, 3 Current issues in molecular biologyUnlocking the Anti-Breast Cancer Potential of <i>Aralia chinensis</i> L.PMC12384973grant numbers Qian Ke He Foundation 2023) General 420Lu TShu YXue JLi LXie CChai HfalseUnlocking the Anti-Breast Cancer Potential of <i>Aralia chinensis</i> L.Aralia chinensis L. has shown potential in breast cancer treatment, yet its pharmacodynamically active components and mechanisms remain undefined. To systematically identify the bioactive constituents absorbed into the bloodstream and elucidate their multi-target mechanisms against breast cancer, we employed ultra-high-performance liquid chromatography in conjunction with Q Exactive Orbitrap mass spectrometry (UHPLC-Q Exactive Orbitrap-MS) alongside serum pharmacochemistry to analyze the chemical constituents of total saponins derived from A. chinensis (TSAC) and to identify the blood-absorbed prototypes in a rat model. Network pharmacology predicted targets and pathways of serum prototypes, validated by molecular docking and in vitro experiments. We identified 38 triterpenoid saponins, 3 2025-01-01T00:00:00Z2025 Aug2026-04-08T11:18:02.327Z2026-04-08T01:52:08.301ZS-EPMC123849734086481610.3390/cimb47080662