biostudies-literatureYang CGuangxi Science and Technology Base and Special TalentsInnovative Team for Traditional Chinese Medicinal Materials Quality of GuangxiNatural Science Foundation of Guangxi ProvinceKey Laboratory Construction Program of Guangxi Health commissionGaungxi Bagui Brilliance Visiting Scholar ProgramGuangxi Traditional Chinese Medicine Appropriate Technology Development and Promotion ProjectNatural Science Foundation of Guangxi Province (2023GXNSFAA026330), Gaungxi Bagui Brilliance Visiting Scholar Program (Lingyun Wan), Guangxi Science and Technology Base and Special Talents (Guike AD22035026), Innovative Team for Traditional Chinese Medici953https://www.ebi.ac.uk/biostudies/studies/S-EPMC12385686biostudies-literatureUnknown16(8)<h4>Background</h4><i>Centella asiatica</i>, a medicinally important species that is rich in bioactive compounds, lacks a characterized mitochondrial genome, despite nuclear and chloroplast assemblies. We sequenced and annotated its mitochondrial genome to elucidate its genetic foundations and evolutionary mechanisms.<h4>Methods</h4>Assembly using Illumina short-reads and Nanopore long-reads was used to characterize the mitochondrial genome. Analyses included structural characterization, codon usage bias, repetitive sequences, horizontal gene transfer (HGT), collinearity, and phylogeny. The resulting tissue-specific (root, stem, and leaf) long non-coding RNA (lncRNA) profiles identified RNA editing sites.<h4>Results</h4>The complete mitochondrial genome (249,777 bp, 45.5% GC) comprises thrGenesMitochondrial Genome and RNA Editing Tissue Specificity of &lt;i&gt;Centella asiatica&lt;/i&gt;.PMC12385686GZKJ2305GZSY23-02(2023GXNSFAA026330); (Guike AD22035026); (GZKJ2305); (GZSY23-02); (ZJC2020003)Guike AD22035026ZJC20200032023GXNSFAA026330Li YLiang WTan GWei SWan LGui LQin YEl-Sappah AHHuang QYang CWei YfalseMitochondrial Genome and RNA Editing Tissue Specificity of &lt;i&gt;Centella asiatica&lt;/i&gt;.<h4>Background</h4><i>Centella asiatica</i>, a medicinally important species that is rich in bioactive compounds, lacks a characterized mitochondrial genome, despite nuclear and chloroplast assemblies. We sequenced and annotated its mitochondrial genome to elucidate its genetic foundations and evolutionary mechanisms.<h4>Methods</h4>Assembly using Illumina short-reads and Nanopore long-reads was used to characterize the mitochondrial genome. Analyses included structural characterization, codon usage bias, repetitive sequences, horizontal gene transfer (HGT), collinearity, and phylogeny. The resulting tissue-specific (root, stem, and leaf) long non-coding RNA (lncRNA) profiles identified RNA editing sites.<h4>Results</h4>The complete mitochondrial genome (249,777 bp, 45.5% GC) comprises thr2025-01-01T00:00:00Z2025 Aug2026-07-05T03:16:25.526Z2026-07-05T03:12:20.103ZS-EPMC123856864087000110.3390/genes16080953