biostudies-literatureKarlsberger LChristian Doppler Research AssociationAustrian Research Promotion Agency322https://www.ebi.ac.uk/biostudies/studies/S-EPMC12387151biostudies-literatureUnknown23(8)Brown algae such as <i>Ascophyllum nodosum</i> (AN) and <i>Fucus vesiculosus</i> (FV) are gaining considerable attention as functional feed additives due to their health-beneficial properties. This study evaluated the antioxidant potential of AN and FV extracts in intestinal epithelial cells and the in vivo model <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Aqueous AN and FV extracts were characterized for total phenolic content (TPC), antioxidant capacity (TEAC, FRAP), and phlorotannin composition using LC-HRMS/MS. Antioxidant effects were assessed in vitro, measuring AAPH-induced ROS production in Caco-2 and IPEC-J2 cells via H₂DCF-DA, and in vivo, evaluating the effects of paraquat-induced oxidative stress and AN or FV treatment on worm motility, GST-4::GFP reporter expMarine drugsAntioxidant Power of Brown Algae: &lt;i&gt;Ascophyllum nodosum&lt;/i&gt; and &lt;i&gt;Fucus vesiculosus&lt;/i&gt; Extracts Mitigate Oxidative Stress In Vitro and In Vivo.PMC12387151905123881882Josef Ressel Center for Phytogenic Drug ResearchWeghuber JMolcanova LKarlsberger LLadirat SSandner GRypar TfalseAntioxidant Power of Brown Algae: &lt;i&gt;Ascophyllum nodosum&lt;/i&gt; and &lt;i&gt;Fucus vesiculosus&lt;/i&gt; Extracts Mitigate Oxidative Stress In Vitro and In Vivo.Brown algae such as <i>Ascophyllum nodosum</i> (AN) and <i>Fucus vesiculosus</i> (FV) are gaining considerable attention as functional feed additives due to their health-beneficial properties. This study evaluated the antioxidant potential of AN and FV extracts in intestinal epithelial cells and the in vivo model <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Aqueous AN and FV extracts were characterized for total phenolic content (TPC), antioxidant capacity (TEAC, FRAP), and phlorotannin composition using LC-HRMS/MS. Antioxidant effects were assessed in vitro, measuring AAPH-induced ROS production in Caco-2 and IPEC-J2 cells via H₂DCF-DA, and in vivo, evaluating the effects of paraquat-induced oxidative stress and AN or FV treatment on worm motility, GST-4::GFP reporter exp2025-01-01T00:00:00Z2025 Aug2026-04-08T15:54:04.567Z2026-04-08T05:44:33.235ZS-EPMC123871514086363910.3390/md23080322