biostudies-literaturede Oliveira PNFundação de Amparo à Pesquisa do Estado de São Paulo3540-3560https://www.ebi.ac.uk/biostudies/studies/S-EPMC12392944biostudies-literatureUnknown23(9)The Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase that plays a crucial role in ubiquitin-dependent proteolysis of key cell cycle regulators, which is completed by the 26S proteasome. Previously, SAMBA, a plant-specific regulator of the APC/C, was identified in Arabidopsis as a critical factor controlling organ size through the regulation of cell proliferation. Here, by assessing its role in the crop tomato (Solanum lycopersicum), we confirm that SAMBA is a conserved APC/C regulator in plants and shows additional roles, including the modulation of fruit shape and changes in sugar metabolism. Two slsamba genome-edited lines were produced and characterized, and showed delayed growth, reduced plant size, and altered fruit morphology, which were linked to changes in celPlant biotechnology journalModulating the activity of the APC/C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits.PMC123929442021/03212-52017/10333‐82020/00888‐52021/06611-82020/00888-52021/06611‐82021/03212‐52017/10333-82023/13107‐02023/13107-0Mauxion JPSaleme MLSFernie ARZhu FMartins MCMChang AIGonzalez NGarcia SSde Oliveira PNRosado-Souza Lda Silva LFCEloy NBFerreira PBDe Beukelaer HBollier NVandepoele KGoossens Ade Souza LPPino LEPeres LEPfalseModulating the activity of the APC/C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits.The Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase that plays a crucial role in ubiquitin-dependent proteolysis of key cell cycle regulators, which is completed by the 26S proteasome. Previously, SAMBA, a plant-specific regulator of the APC/C, was identified in Arabidopsis as a critical factor controlling organ size through the regulation of cell proliferation. Here, by assessing its role in the crop tomato (Solanum lycopersicum), we confirm that SAMBA is a conserved APC/C regulator in plants and shows additional roles, including the modulation of fruit shape and changes in sugar metabolism. Two slsamba genome-edited lines were produced and characterized, and showed delayed growth, reduced plant size, and altered fruit morphology, which were linked to changes in cel2025-01-01T00:00:00Z2025 Sep2026-05-27T21:12:37.538Z2026-04-08T01:50:31.587ZS-EPMC123929444048358210.1111/pbi.70149