biostudies-literatureUnknown12Yu HL<h4>Introduction</h4>The chicken gut microbiome plays a pivotal role in nutrient absorption and overall health, contributing to the biosynthesis of essential vitamins. However, the biosynthesis of vitamins B and K<sub>2</sub> by the whole gut microbiome, as well as their abundances across different gut regions, remains largely unknown.<h4>Methods</h4>We employed both metagenomic sequencing and culture-based techniques, collecting a total of 25,825 genomes (25,764 metagenome-assembled genomes and 61 isolated genomes). After quality assessment and average nucleotide identity (ANI), 13,734 genomes were retained for downstream analysis.<h4>Results</h4>Whole-genome clustering analysis identified 2,675 species-level genome bins (SGBs), predominantly from the phyla <i>Bacillota, Bacteroidota, PseFrontiers in veterinary science1646825https://www.ebi.ac.uk/biostudies/studies/S-EPMC12393000biostudies-literatureInsights from metagenomics on microbial biosynthesis of vitamins B and K&lt;sub&gt;2&lt;/sub&gt; in chicken gut microbiota.PMC12393000Zhao JXZhang XXLiu JWei YJLiu GHNi HBChen BNNan FLLiu RMeng JXShang KMYang XYu HLMa HCai YHou XWfalseInsights from metagenomics on microbial biosynthesis of vitamins B and K&lt;sub&gt;2&lt;/sub&gt; in chicken gut microbiota.<h4>Introduction</h4>The chicken gut microbiome plays a pivotal role in nutrient absorption and overall health, contributing to the biosynthesis of essential vitamins. However, the biosynthesis of vitamins B and K<sub>2</sub> by the whole gut microbiome, as well as their abundances across different gut regions, remains largely unknown.<h4>Methods</h4>We employed both metagenomic sequencing and culture-based techniques, collecting a total of 25,825 genomes (25,764 metagenome-assembled genomes and 61 isolated genomes). After quality assessment and average nucleotide identity (ANI), 13,734 genomes were retained for downstream analysis.<h4>Results</h4>Whole-genome clustering analysis identified 2,675 species-level genome bins (SGBs), predominantly from the phyla <i>Bacillota, Bacteroidota, Pse2025-01-01T00:00:00Z20252026-04-08T15:39:43.917Z2026-04-08T05:17:23.066ZS-EPMC123930004089579210.3389/fvets.2025.1646825