{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yamada D"],"funding":["MEXT | Japan Science and Technology Agency (JST)","MEXT | Japan Society for the Promotion of Science (JSPS)"],"pagination":["256"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12397333"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Ultraviolet (UV) irradiation of DNA causes genotoxic photolesions, such as carcinogenic pyrimidine(6-4)pyrimidone photoproducts ((6-4)PPs). In many organisms, (6-4)PPs are repaired by (6-4) photolyases, which contain a flavin chromophore and use blue light energy to initiate the catalytic reaction. Although (6-4)PP repair has been shown to require the input of two successive photons, details of the mechanism remain elusive. Here, we applied recently developed time-resolved UV and infrared (IR) spectroscopic techniques to capture a key intermediate that awaits the second photon in the repair reaction of Xenopus laevis (6-4) photolyase. The results indicate that the intermediate is formed within 500 μs following the initial absorption of a photon and contains a four-membered oxetane ring tha"],"journal":["Communications chemistry"],"pubmed_title":["Infrared and ultraviolet spectroscopic characterization of a key intermediate during DNA repair by (6-4) photolyase."],"pmcid":["PMC12397333"],"funding_grant_id":["JP19H05784","JP22H04751","JP20H05442","JP24H02263","JPMJFR2057"],"pubmed_authors":["Hamamura T","Yanagisawa S","Yamada D","Yamamoto J","Shigeta Y","Kadono A","Maeno T","Kubo M","Sato W"],"additional_accession":[]},"is_claimable":false,"name":"Infrared and ultraviolet spectroscopic characterization of a key intermediate during DNA repair by (6-4) photolyase.","description":"Ultraviolet (UV) irradiation of DNA causes genotoxic photolesions, such as carcinogenic pyrimidine(6-4)pyrimidone photoproducts ((6-4)PPs). In many organisms, (6-4)PPs are repaired by (6-4) photolyases, which contain a flavin chromophore and use blue light energy to initiate the catalytic reaction. Although (6-4)PP repair has been shown to require the input of two successive photons, details of the mechanism remain elusive. Here, we applied recently developed time-resolved UV and infrared (IR) spectroscopic techniques to capture a key intermediate that awaits the second photon in the repair reaction of Xenopus laevis (6-4) photolyase. The results indicate that the intermediate is formed within 500 μs following the initial absorption of a photon and contains a four-membered oxetane ring tha","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-07-15T14:15:16.934Z","creation":"2026-07-05T03:12:31.252Z"},"accession":"S-EPMC12397333","cross_references":{"pubmed":["40883597"],"doi":["10.1038/s42004-025-01625-9"]}}