{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Doyle LP"],"funding":["Sylvester Comprehensive Cancer Center","NIH National Institute of General Medical Sciences","National Cancer Institute","NCI NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["113298"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12397940"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(9)"],"pubmed_abstract":["Cdc42 is a Rho-family GTPase that controls cell polarization from yeast to human cells. In fission yeast, under normal growth conditions, Cdc42-GTP oscillates between cell tips to promote polarized growth. However, when exposed to environmental stressors, Cdc42 adopts an \"exploratory\" pattern of Cdc42 activation along the cell membrane. This pattern also occurs when the NDR kinase Orb6 is downregulated. Here, we describe the molecular mechanism behind the emergence of exploratory Cdc42 dynamics and identify a substrate of Orb6 kinase, the Cdc42 GAP Rga3. Additionally, we show that MAP kinase Sty1, known for linking stress signals to the Cdc42 polarity module, negatively regulates Orb6 kinase. During nutritional stress, activation of Sty1 and inactivation of Orb6 are associated with chronol"],"journal":["iScience"],"pubmed_title":["Control of stress-activated Cdc42 dynamics by the MAP kinase Sty1-NDR kinase Orb6 regulatory axis."],"pmcid":["PMC12397940"],"funding_grant_id":["R01 GM129514","P30CA240139","P30 CA240139"],"pubmed_authors":["Chen JS","Gould KL","Doyle LP","McCollum D","Verde F"],"additional_accession":[]},"is_claimable":false,"name":"Control of stress-activated Cdc42 dynamics by the MAP kinase Sty1-NDR kinase Orb6 regulatory axis.","description":"Cdc42 is a Rho-family GTPase that controls cell polarization from yeast to human cells. In fission yeast, under normal growth conditions, Cdc42-GTP oscillates between cell tips to promote polarized growth. However, when exposed to environmental stressors, Cdc42 adopts an \"exploratory\" pattern of Cdc42 activation along the cell membrane. This pattern also occurs when the NDR kinase Orb6 is downregulated. Here, we describe the molecular mechanism behind the emergence of exploratory Cdc42 dynamics and identify a substrate of Orb6 kinase, the Cdc42 GAP Rga3. Additionally, we show that MAP kinase Sty1, known for linking stress signals to the Cdc42 polarity module, negatively regulates Orb6 kinase. During nutritional stress, activation of Sty1 and inactivation of Orb6 are associated with chronol","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-02T20:17:43.011Z","creation":"2026-04-20T03:10:08.358Z"},"accession":"S-EPMC12397940","cross_references":{"pubmed":["40894867"],"doi":["10.1016/j.isci.2025.113298"]}}