biostudies-literatureJin Ythe Yan Xiaohua Fujian Provincial Famous Traditional Chinese Medicine Expert Inheritance Studiothe Construction Program for Inheritance Office of National Famous TCM Expert Yan XiaohuaScience and Technology Innovation Joint Fund Project of the Department of Science and Technology of Fujian Provincethe Natural Science Foundation of Fujian Provincethe Project for the Construction of Flagship Hospitals for the Collaboration of Traditional Chinese and Western Medicine178https://www.ebi.ac.uk/biostudies/studies/S-EPMC12398093biostudies-literatureUnknown162(1)Cancer cachexia, a debilitating syndrome characterized by muscle wasting and systemic inflammation, remains a major unmet clinical need. Qingxie Fuzheng Granules (QFG), a traditional Chinese medicine formulation, have shown promise in cancer therapy, but their role in cachexia management is unclear.Here, we investigated the anti-cachectic effects of QFG in a murine model of colon adenocarcinoma-induced cachexia. 16S rRNA sequencing revealed gut dysbiosis in cachectic mice, with increased Enterobacteriaceae and decreased Lactobacillus. QFG treatment restored microbial balance, reduced pro-inflammatory cytokines TNF-α, IL-6, and enhanced intestinal barrier integrity by upregulating tight junction proteins ZO-1, Occludin, Calprotectin.Mechanistically, QFG rebalanced Th17/Treg cell ratios and HereditasQingxie Fuzheng granules attenuate cancer cachexia by restoring gut microbiota homeostasis and suppressing IL-6/NF-κB signaling in colorectal adenocarcinoma.PMC12398093Grant No. 2023-697Grant No. 2023J01884No.2022-752024Y9496Lu LHua HFang WRen PChen WGeng ZLin JJin YLin KChen BYan XWang LfalseQingxie Fuzheng granules attenuate cancer cachexia by restoring gut microbiota homeostasis and suppressing IL-6/NF-κB signaling in colorectal adenocarcinoma.Cancer cachexia, a debilitating syndrome characterized by muscle wasting and systemic inflammation, remains a major unmet clinical need. Qingxie Fuzheng Granules (QFG), a traditional Chinese medicine formulation, have shown promise in cancer therapy, but their role in cachexia management is unclear.Here, we investigated the anti-cachectic effects of QFG in a murine model of colon adenocarcinoma-induced cachexia. 16S rRNA sequencing revealed gut dysbiosis in cachectic mice, with increased Enterobacteriaceae and decreased Lactobacillus. QFG treatment restored microbial balance, reduced pro-inflammatory cytokines TNF-α, IL-6, and enhanced intestinal barrier integrity by upregulating tight junction proteins ZO-1, Occludin, Calprotectin.Mechanistically, QFG rebalanced Th17/Treg cell ratios and 2025-01-01T00:00:00Z2025 Aug2026-04-08T19:32:43.39Z2026-04-08T13:45:23.15ZS-EPMC123980934088385510.1186/s41065-025-00541-1