{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee WJ"],"funding":["Ministry of Education","National Research Foundation of Korea"],"pagination":["215-227"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12400079"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(3)"],"pubmed_abstract":["Clonorchis sinensis is a liver fluke that causes clonorchiasis, a significant public health concern in East Asia, closely associated with hepatobiliary diseases. Dopamine is an essential neurotransmitter involved in neuromuscular signaling, and its uptake by trematodes may contribute to parasite physiology and survival. This study aimed to characterize the dopamine transporter CsDAT in C. sinensis by synthesizing cDNA from adult worms and expressing it in Xenopus laevis oocytes; subsequently, uptake assays were conducted using radiolabeled dopamine. Functional assays confirmed that CsDAT mediates dopamine uptake in a sodium-dependent manner. The uptake was saturable and exhibited Michaelis-Menten kinetics with a Michaelis constant of 454.5 nM and a maximum uptake rate of 1,422.5 fmol/oocyt"],"journal":["Parasites, hosts and diseases"],"pubmed_title":["Clonorchis sinensis dopamine transporter (CsDAT) facilitates dopamine uptake."],"pmcid":["PMC12400079"],"funding_grant_id":["RS-2023-00240627","2017R1D1A1B03034588"],"pubmed_authors":["Cha SH","Lee WK","Lee WJ","Kim SJ","Han JH"],"additional_accession":[]},"is_claimable":false,"name":"Clonorchis sinensis dopamine transporter (CsDAT) facilitates dopamine uptake.","description":"Clonorchis sinensis is a liver fluke that causes clonorchiasis, a significant public health concern in East Asia, closely associated with hepatobiliary diseases. Dopamine is an essential neurotransmitter involved in neuromuscular signaling, and its uptake by trematodes may contribute to parasite physiology and survival. This study aimed to characterize the dopamine transporter CsDAT in C. sinensis by synthesizing cDNA from adult worms and expressing it in Xenopus laevis oocytes; subsequently, uptake assays were conducted using radiolabeled dopamine. Functional assays confirmed that CsDAT mediates dopamine uptake in a sodium-dependent manner. The uptake was saturable and exhibited Michaelis-Menten kinetics with a Michaelis constant of 454.5 nM and a maximum uptake rate of 1,422.5 fmol/oocyt","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-07-15T09:04:07.657Z","creation":"2026-07-02T03:08:40.806Z"},"accession":"S-EPMC12400079","cross_references":{"pubmed":["40888015"],"doi":["10.3347/PHD.25040"]}}