biostudies-literatureUnknown13(9)Chen JDiseases triggered by phytopathogenic bacteria lead to substantial economic losses within the realm of agricultural production. <i>Bacillus velezensis</i> is an important agricultural biocontrol and plant growth-promoting bacterium. In this study, we evaluated the inhibitory ability of <i>B. velezensis</i> D83 against eight plant pathogenic fungi. D83 has a broad spectrum of antagonistic activity against these eight plant pathogens. In addition, our study found that D83 promotes seed germination and plant growth in maize and tobacco, and this growth-promoting ability is related to its growth-promoting properties such as indoleacetic acid production, nitrogen fixation, and phosphate solubilization. This effect of promoting plant growth is more effective than the microbial preparations on thMicrobiology spectrume0030025https://www.ebi.ac.uk/biostudies/studies/S-EPMC12403654biostudies-literatureGenomics and metabolomics assisted functional characterization of <i>Bacillus velezensis</i> D83 as a biocontrol and plant growth-promoting bacterium.PMC12403654Trung Tin HChen JXiao ZDong JJiang DYu JLiu THung Tien LZhao XfalseGenomics and metabolomics assisted functional characterization of <i>Bacillus velezensis</i> D83 as a biocontrol and plant growth-promoting bacterium.Diseases triggered by phytopathogenic bacteria lead to substantial economic losses within the realm of agricultural production. <i>Bacillus velezensis</i> is an important agricultural biocontrol and plant growth-promoting bacterium. In this study, we evaluated the inhibitory ability of <i>B. velezensis</i> D83 against eight plant pathogenic fungi. D83 has a broad spectrum of antagonistic activity against these eight plant pathogens. In addition, our study found that D83 promotes seed germination and plant growth in maize and tobacco, and this growth-promoting ability is related to its growth-promoting properties such as indoleacetic acid production, nitrogen fixation, and phosphate solubilization. This effect of promoting plant growth is more effective than the microbial preparations on th2025-01-01T00:00:00Z2025 Sep2026-05-29T17:32:31.519Z2026-04-08T05:37:22.93ZS-EPMC124036544076786110.1128/spectrum.00300-25