{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Sewall LM"],"funding":["NIAID NIH HHS","Gates Foundation"],"pubmed_abstract":["Understanding the mechanistic interplay between antibodies and invading pathogens is essential for vaccine development. Current methods are labour and time intensive and limited by sample preparation bottlenecks. Here we present microfluidic electron microscopy-based polyclonal epitope mapping (mEM), which combines microfluidics with single-particle electron microscopy for the structural characterization of immune complexes using small volumes of sera (<4 µl). First, we used mEM to map polyclonal antibodies present in sera from infected and vaccinated individuals against five viral glycoproteins using negative-stain electron microscopy. The mEM detected a greater number of epitopes compared with conventional polyclonal epitope structural mapping methods. Second, we used mEM and cryo-electr"],"journal":["Nature biomedical engineering"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12404239"],"repository":["biostudies-literature"],"pubmed_title":["Microfluidics combined with electron microscopy for rapid and high-throughput mapping of antibody-viral glycoprotein complexes."],"pmcid":["PMC12404239"],"funding_grant_id":["INV-002916","UM1 AI144462","R01 AI136621"],"pubmed_authors":["Mohanty S","Xie Z","Bangaru S","Beutler N","Torrents de la Pena A","Burton DR","Sewall LM","Ozorowski G","Rogers TF","Batista FD","Chocarro Ruiz B","Tran AS","de Paiva Froes Rocha R","Gibson G","Shaw AC","Ward AB","Louie M"],"additional_accession":[]},"is_claimable":false,"name":"Microfluidics combined with electron microscopy for rapid and high-throughput mapping of antibody-viral glycoprotein complexes.","description":"Understanding the mechanistic interplay between antibodies and invading pathogens is essential for vaccine development. Current methods are labour and time intensive and limited by sample preparation bottlenecks. Here we present microfluidic electron microscopy-based polyclonal epitope mapping (mEM), which combines microfluidics with single-particle electron microscopy for the structural characterization of immune complexes using small volumes of sera (<4 µl). First, we used mEM to map polyclonal antibodies present in sera from infected and vaccinated individuals against five viral glycoproteins using negative-stain electron microscopy. The mEM detected a greater number of epitopes compared with conventional polyclonal epitope structural mapping methods. Second, we used mEM and cryo-electr","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-05-29T15:15:30.621Z","creation":"2026-04-08T05:11:53.58Z"},"accession":"S-EPMC12404239","cross_references":{"pubmed":["40461656"],"doi":["10.1038/s41551-025-01411-x"]}}