biostudies-literatureUnknown5(1)Martinez-Garcia MMicrobes and their viruses drive central biogeochemical cycles on a global scale. Understanding the biology and ecology of virus-host interactions and their impact on ecosystems depends on our ability to develop tools that enable high-throughput screening of ecologically relevant, uncultured virus-host pairs. Viruses infecting Pelagibacterales, the predominant bacteria in surface oceans, have been studied through computational analyses and cultivation efforts. Here, we employ an accessible microfluidics and semi-permeable capsule (SPC) technology to investigate the uncultured pelagiphage vSAG 37-F6-host interactions since it is one of the most abundant and ubiquitous viruses in the marine virosphere. First, we validated this technology using cultured virus-host pairs. Then, marine single cISME communicationsycaf123https://www.ebi.ac.uk/biostudies/studies/S-EPMC12404659biostudies-literatureTargeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology.PMC12404659Anton JPerez-Martin LLluesma-Gomez MSanchez FSOrphan VNadal-Molero FRubio-Portillo EMartin-Cuadrado ABEscolano-Vico AMartinez-Garcia MfalseTargeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology.Microbes and their viruses drive central biogeochemical cycles on a global scale. Understanding the biology and ecology of virus-host interactions and their impact on ecosystems depends on our ability to develop tools that enable high-throughput screening of ecologically relevant, uncultured virus-host pairs. Viruses infecting Pelagibacterales, the predominant bacteria in surface oceans, have been studied through computational analyses and cultivation efforts. Here, we employ an accessible microfluidics and semi-permeable capsule (SPC) technology to investigate the uncultured pelagiphage vSAG 37-F6-host interactions since it is one of the most abundant and ubiquitous viruses in the marine virosphere. First, we validated this technology using cultured virus-host pairs. Then, marine single c2025-01-01T00:00:00Z2025 Jan2026-05-29T21:32:31.111Z2026-04-08T06:03:19.323ZS-EPMC124046594090454110.1093/ismeco/ycaf123