biostudies-literatureKuang Hlnstitutional Research Fund of Sichuan UniversityScience and Technology Department of Sichuan ProvinceNational Natural Science Foundation of China84https://www.ebi.ac.uk/biostudies/studies/S-EPMC12405079biostudies-literatureUnknown18(1)The 14-3-3 proteins are highly conserved and widely distributed across eukaryotes. Some 14-3-3 proteins have been identified as regulators of phosphorus (Pi) deficiency tolerance in rice, but their diverse functions remain largely unexplored. In this study, we characterized the role of rice plant-specific non-ε group 14-3-3 proteins (OsGF14a-f) in response to Pi starvation by mutating these genes. We found that the expression of OsGF14a decreased in response to Pi starvation, while the expression of other non-ε group genes was induced. Subcellular localization studies transiently expressing them in tobacco leaves showed that OsGF14a was present in both the cytoplasm and nucleus, whereas the other proteins were predominantly localized in the cytoplasm. By developing single and multiple mutaRice (New York, N.Y.)Genetic Dissection of Rice Plant-Specific Non-ε Group 14-3-3 Proteins in Response to Phosphate Starvation.PMC124050792020SCUNL21232072659U22A204942021ZDZX0012Yan MDeng TYang JZhu YChen WXie MLin HKuang HChen SWang ZfalseGenetic Dissection of Rice Plant-Specific Non-ε Group 14-3-3 Proteins in Response to Phosphate Starvation.The 14-3-3 proteins are highly conserved and widely distributed across eukaryotes. Some 14-3-3 proteins have been identified as regulators of phosphorus (Pi) deficiency tolerance in rice, but their diverse functions remain largely unexplored. In this study, we characterized the role of rice plant-specific non-ε group 14-3-3 proteins (OsGF14a-f) in response to Pi starvation by mutating these genes. We found that the expression of OsGF14a decreased in response to Pi starvation, while the expression of other non-ε group genes was induced. Subcellular localization studies transiently expressing them in tobacco leaves showed that OsGF14a was present in both the cytoplasm and nucleus, whereas the other proteins were predominantly localized in the cytoplasm. By developing single and multiple muta2025-01-01T00:00:00Z2025 Sep2026-05-29T21:45:16.818Z2026-04-08T06:08:31.612ZS-EPMC124050794089231110.1186/s12284-025-00840-1