biostudies-literatureGarcia-Marquez JJuan de la CiervaAgencia Estatal de InvestigaciónJunta de AndalucíaUniversidad de Málaga2179-2196https://www.ebi.ac.uk/biostudies/studies/S-EPMC12405380biostudies-literatureUnknown17(4)This study investigates the postbiotic potential of extracellular products (ECPs) from Bacillus pumilus strains cultivated on microalgae-supplemented media. We assessed enzymatic and antimicrobial activities to select ECPs that enhance the digestive processes in gilthead seabream. Additionally, we explored the in vitro enzymatic capacity of the chosen postbiotics to hydrolyze macromolecules in microalgae. Finally, a feeding trial was conducted to determine the in vivo effects of the ECPs on Sparus aurata. In vitro enzymatic assays demonstrated diverse hydrolytic capacities among ECPs. All conditions exhibited antimicrobial activity against Photobacterium damselae subsp. piscicida, with variation in inhibitory effects against Vibrio harveyi and Tenacibaculum maritimum. Furthermore, in vitroProbiotics and antimicrobial proteinsMicroalgal and Cyanobacterial Biomasses Modified the Activity of Extracellular Products from Bacillus pumilus: An In Vitro and In Vivo Assessment.PMC12405380P20_00085PID2020-113637RB-C22PID2021-12228OBC21FJCI-2021-047759-IFEDER-UCA18-107182Garcia-Marquez JMartos-Sitcha JAMorinigo MABalebona MCDiaz AGMolina-Roque LVizcaino AJAlarcon-Lopez FJSimo-Mirabet PDominguez-Maqueda Mde Las Heras VfalseMicroalgal and Cyanobacterial Biomasses Modified the Activity of Extracellular Products from Bacillus pumilus: An In Vitro and In Vivo Assessment.This study investigates the postbiotic potential of extracellular products (ECPs) from Bacillus pumilus strains cultivated on microalgae-supplemented media. We assessed enzymatic and antimicrobial activities to select ECPs that enhance the digestive processes in gilthead seabream. Additionally, we explored the in vitro enzymatic capacity of the chosen postbiotics to hydrolyze macromolecules in microalgae. Finally, a feeding trial was conducted to determine the in vivo effects of the ECPs on Sparus aurata. In vitro enzymatic assays demonstrated diverse hydrolytic capacities among ECPs. All conditions exhibited antimicrobial activity against Photobacterium damselae subsp. piscicida, with variation in inhibitory effects against Vibrio harveyi and Tenacibaculum maritimum. Furthermore, in vitro2025-01-01T00:00:00Z2025 Aug2026-05-29T21:40:59.051Z2026-04-08T06:05:33.659ZS-EPMC124053803925937710.1007/s12602-024-10350-z