biostudies-literatureHuang JCanada Research Chairs Program of the Natural Sciences and Engineering Research CouncilNew Frontiers in Research FundNatural Sciences and Engineering Research Council Canada Graduate Scholarship Doctoral ProgramCanadian Institutes of Health Research29https://www.ebi.ac.uk/biostudies/studies/S-EPMC12408339biostudies-literatureUnknown2(1)We report a synthetic tetrahedral DNA nanocarrier (TDN) for treating bone defects and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infection using in vitro studies. We successfully synthesized TDNs and demonstrated their excellent cytocompatibility with blood cells and immune cells. Zoledronic acid-loaded TDN displayed increased efficacy compared to free drugs in regulating bone remodeling, while vancomycin-loaded TDN showed an increased antibacterial effect against MRSA. In the future, this nano-drug delivery platform, with its multifunctional properties, can be potentially used to treat bone fractures and surgery-related MRSA infection.NPJ biomedical innovationsSelf-assembled DNA nanocarrier-enabled drug delivery for bone remodeling and antimicrobial applications.PMC12408339NFRFE-2019-00743185629CRC-2018-00028Huang JZahid AALuo WPaul AChakraborty AShamiya YfalseSelf-assembled DNA nanocarrier-enabled drug delivery for bone remodeling and antimicrobial applications.We report a synthetic tetrahedral DNA nanocarrier (TDN) for treating bone defects and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infection using in vitro studies. We successfully synthesized TDNs and demonstrated their excellent cytocompatibility with blood cells and immune cells. Zoledronic acid-loaded TDN displayed increased efficacy compared to free drugs in regulating bone remodeling, while vancomycin-loaded TDN showed an increased antibacterial effect against MRSA. In the future, this nano-drug delivery platform, with its multifunctional properties, can be potentially used to treat bone fractures and surgery-related MRSA infection.2025-01-01T00:00:00Z20252026-06-01T13:48:57.118Z2026-04-08T13:11:06.5ZS-EPMC124083394091768010.1038/s44385-025-00020-2