biostudies-literatureLee SYMinistry of Science and ICT, South KoreaNational Research Foundation of Koreae2505019https://www.ebi.ac.uk/biostudies/studies/S-EPMC12409433biostudies-literatureUnknown35N-acetylglucosamine-6-phosphate deacetylase (NagA) is a conserved enzyme involved in bacterial amino sugar metabolism, catalyzing the conversion of GlcNAc-6-phosphate to GlcN-6-phosphate and acetate. While NagA typically function as dimers, its quaternary diversity across species remains underexplored. Here, we present the crystal structure of <i>Klebsiella pneumoniae</i> (kpNagA), which forms a homotetrameric assembly both in crystal and in solution, as confirmed by SEC-MALS. Each monomer adopts a canonical (β/α)<sub>8</sub> TIM barrel fold with a β-sandwich subdomain, and its active site, located around β10-β11 and α3-α4, coordinates a divalent zinc ion. Comparative analyses revealed conserved dimer interfaces but divergent tetrameric arrangements. Notably, <i>Pasteurella multocida</i> NJournal of microbiology and biotechnologyStructural Basis for Tetramerization of <i>Klebsiella pneumoniae</i> <i>N</i>-Acetylglucosamine-6-Phosphate Deacetylase.PMC12409433RS-2025-02316334Lee SYPark HHfalseStructural Basis for Tetramerization of <i>Klebsiella pneumoniae</i> <i>N</i>-Acetylglucosamine-6-Phosphate Deacetylase.N-acetylglucosamine-6-phosphate deacetylase (NagA) is a conserved enzyme involved in bacterial amino sugar metabolism, catalyzing the conversion of GlcNAc-6-phosphate to GlcN-6-phosphate and acetate. While NagA typically function as dimers, its quaternary diversity across species remains underexplored. Here, we present the crystal structure of <i>Klebsiella pneumoniae</i> (kpNagA), which forms a homotetrameric assembly both in crystal and in solution, as confirmed by SEC-MALS. Each monomer adopts a canonical (β/α)<sub>8</sub> TIM barrel fold with a β-sandwich subdomain, and its active site, located around β10-β11 and α3-α4, coordinates a divalent zinc ion. Comparative analyses revealed conserved dimer interfaces but divergent tetrameric arrangements. Notably, <i>Pasteurella multocida</i> N2025-01-01T00:00:00Z2025 Aug2026-05-29T21:41:28.471Z2026-04-08T06:06:11.965ZS-EPMC124094334087701910.4014/jmb.2505.05019