biostudies-literatureHawkes JASvenska Forskningsr?det FormasUppsala Universitet18612-18620https://www.ebi.ac.uk/biostudies/studies/S-EPMC12409697biostudies-literatureUnknown97(34)Dissolved organic matter (DOM) is one of the most complex chemical mixtures known, with its chemical composition having long puzzled biogeochemists. Identifying the chemical structures within DOM is essential for unraveling its origins and environmental fate. However, DOM's complexity has impeded structural elucidation, and molecules with accurate functional group compositions for recalcitrant DOM are poorly represented in the synthetic and isolative literature. Consequently, hypothesized DOM compounds are derived from models that inadequately represent true structures. To address this, carboxylic-acid-only CRAM analogues were previously synthesized but failed to replicate the extensive fragmentation observed in marine DOM during tandem mass spectrometry (MS2). Here, we prepared CRAM analoAnalytical chemistryEnhanced Structural Understanding of Dissolved Organic Matter through Comparative LC/MS2 Analysis with Synthetic Carboxylate Rich Alicyclic Molecules.PMC124096972021-00543Moodie LWKCraig AJHawkes JAFlygare ADfalseEnhanced Structural Understanding of Dissolved Organic Matter through Comparative LC/MS2 Analysis with Synthetic Carboxylate Rich Alicyclic Molecules.Dissolved organic matter (DOM) is one of the most complex chemical mixtures known, with its chemical composition having long puzzled biogeochemists. Identifying the chemical structures within DOM is essential for unraveling its origins and environmental fate. However, DOM's complexity has impeded structural elucidation, and molecules with accurate functional group compositions for recalcitrant DOM are poorly represented in the synthetic and isolative literature. Consequently, hypothesized DOM compounds are derived from models that inadequately represent true structures. To address this, carboxylic-acid-only CRAM analogues were previously synthesized but failed to replicate the extensive fragmentation observed in marine DOM during tandem mass spectrometry (MS2). Here, we prepared CRAM analo2025-01-01T00:00:00Z2025 Sep2026-05-29T20:11:11.413Z2026-04-08T05:59:29.667ZS-EPMC124096974083963410.1021/acs.analchem.5c02665