{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bae HR"],"funding":["National Research Foundation of Korea"],"pagination":["297"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12411337"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["74(9)"],"pubmed_abstract":["Although checkpoint immunotherapy has primarily focused on CD8⁺ T cells, emerging evidence highlights an important role for cytotoxic CD4⁺ T cells in mediating therapeutic responses. However, research on the functional properties of cytotoxic CD4⁺ T cells in the context of immunotherapy is still at an early stage and remains insufficiently defined. Utilizing single-cell RNA-sequencing datasets obtained from metastatic melanoma patients treated with checkpoint inhibitors targeting PD-1 and/or CTLA-4, we performed transcriptomic profiling of conventional CD4⁺ T cells, excluding proliferative and regulatory (FOXP3⁺) subsets, and compared responders and non-responders as distinct groups. Importantly, our analysis identified distinct clusters that discriminate between responders and non-respond"],"journal":["Cancer immunology, immunotherapy : CII"],"pubmed_title":["Cytotoxic CD4⁺ T cells exhibit an immunosuppressive shift in checkpoint immunotherapy resistance in melanoma patients."],"pmcid":["PMC12411337"],"funding_grant_id":["RS-2024-00342528","RS-2023-00213596"],"pubmed_authors":["Kim S","Bae HR","Son B","Young HA","Kwon EY","Hwang K"],"additional_accession":[]},"is_claimable":false,"name":"Cytotoxic CD4⁺ T cells exhibit an immunosuppressive shift in checkpoint immunotherapy resistance in melanoma patients.","description":"Although checkpoint immunotherapy has primarily focused on CD8⁺ T cells, emerging evidence highlights an important role for cytotoxic CD4⁺ T cells in mediating therapeutic responses. However, research on the functional properties of cytotoxic CD4⁺ T cells in the context of immunotherapy is still at an early stage and remains insufficiently defined. Utilizing single-cell RNA-sequencing datasets obtained from metastatic melanoma patients treated with checkpoint inhibitors targeting PD-1 and/or CTLA-4, we performed transcriptomic profiling of conventional CD4⁺ T cells, excluding proliferative and regulatory (FOXP3⁺) subsets, and compared responders and non-responders as distinct groups. Importantly, our analysis identified distinct clusters that discriminate between responders and non-respond","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-29T19:43:38.688Z","creation":"2026-04-08T05:50:29.448Z"},"accession":"S-EPMC12411337","cross_references":{"pubmed":["40906211"],"doi":["10.1007/s00262-025-04145-6"]}}