biostudies-literatureUnknownHeiniger EKNational Institutes of HealthCurable sexually transmitted infections (STIs) caused by the bacteria <i>Chlamydia trachomatis</i> (CT) and <i>Neisseria gonorrhoeae</i> (NG) are widespread globally. These infections are particularly dangerous for female patients, causing pelvic inflammatory disease, infertility, and increased risk of HIV acquisition. Vaginal self-swab sampling can improve access to STI screening but is still subject to treatment delays due to centralized processing. A low-cost point-of-care (POC) device capable of detecting these bacteria from a self- or clinician-collected vaginal swab could address this delay and allow for more timely treatment. In this work, vaginal swab materials from patients infected with CT or NG required a filtration step before lysis and loop-mediated isothermal amplification (LThe Analysthttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12412115biostudies-literatureA low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.PMC12412115U54EB007958Jiang KPYager PHeiniger EKKumar SfalseA low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.Curable sexually transmitted infections (STIs) caused by the bacteria <i>Chlamydia trachomatis</i> (CT) and <i>Neisseria gonorrhoeae</i> (NG) are widespread globally. These infections are particularly dangerous for female patients, causing pelvic inflammatory disease, infertility, and increased risk of HIV acquisition. Vaginal self-swab sampling can improve access to STI screening but is still subject to treatment delays due to centralized processing. A low-cost point-of-care (POC) device capable of detecting these bacteria from a self- or clinician-collected vaginal swab could address this delay and allow for more timely treatment. In this work, vaginal swab materials from patients infected with CT or NG required a filtration step before lysis and loop-mediated isothermal amplification (L2025-01-01T00:00:00Z2025 Sep2026-05-29T21:42:49.634Z2026-04-08T06:05:30.326ZS-EPMC124121154091037410.1039/d5an00496a