{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kurauskas V"],"funding":["NIH National Institute of General Medical Sciences","National Institute of Allergy and Infectious Diseases Division of Microbiology and Infectious Diseases","NCRR NIH HHS","NIAID NIH HHS","University of Wisconsin-Madison","National Institutes of Health","NIGMS NIH HHS","National Science Foundation"],"pagination":["169400"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12412191"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["437(21)"],"pubmed_abstract":["The coronavirus genome is transcribed by a replication-transcription complex (RTC) containing the RNA polymerase plus additional cofactors. The cofactor nsp8 is an important component of the RTC in both alpha and betacoronaviruses required for nsp12 polymerase activity, complex stability, and recruitment of other RTC cofactors. Here we use NMR and other biophysical methods to characterize the structural features and oligomeric state of full-length nsp8 in solution. We show that the C-terminal domain of nsp8 has molten-globule like intrinsic disorder, while the N-terminal domain retains its folded structure in the absence of binding partners. Our data also shows a concentration-dependent association of nsp8 into dimers and possibly tetramers, but not larger molecular weight species. Upon bi"],"journal":["Journal of molecular biology"],"pubmed_title":["The C-terminal Domain of SARS-CoV-2 nsp8 is a Molten Globule in the Absence of Binding Partners."],"pmcid":["PMC12412191"],"funding_grant_id":["R24GM141526","EAGER MCB-2031269","R24 GM141526","R35 GM141748","BIR-9512577","R35GM141748","R01 AI158463","S10 RR13790","R01AI158463","S10 RR013790"],"pubmed_authors":["Henzler-Wildman K","Kirchdoerfer RN","Tonelli M","Kurauskas V"],"additional_accession":[]},"is_claimable":false,"name":"The C-terminal Domain of SARS-CoV-2 nsp8 is a Molten Globule in the Absence of Binding Partners.","description":"The coronavirus genome is transcribed by a replication-transcription complex (RTC) containing the RNA polymerase plus additional cofactors. The cofactor nsp8 is an important component of the RTC in both alpha and betacoronaviruses required for nsp12 polymerase activity, complex stability, and recruitment of other RTC cofactors. Here we use NMR and other biophysical methods to characterize the structural features and oligomeric state of full-length nsp8 in solution. We show that the C-terminal domain of nsp8 has molten-globule like intrinsic disorder, while the N-terminal domain retains its folded structure in the absence of binding partners. Our data also shows a concentration-dependent association of nsp8 into dimers and possibly tetramers, but not larger molecular weight species. Upon bi","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-02T05:22:22.552Z","creation":"2026-04-14T03:13:50.818Z"},"accession":"S-EPMC12412191","cross_references":{"pubmed":["40840586"],"doi":["10.1016/j.jmb.2025.169400"]}}