biostudies-literatureXu YDeutscher Akademischer Austauschdienst (DAAD) and University Medicine Greifswald, AnschubfinanzierungDeutsche ForschungsgemeinschaftNational Natural Science Foundation of ChinaGerman Ministry of Education and ResearchShanghai Pujiang ProgramShanghai Science and Technology Innovation Natural Foundatione01175https://www.ebi.ac.uk/biostudies/studies/S-EPMC12412467biostudies-literatureUnknown12(33)Chronic kidney disease (CKD) is characterized by persistent inflammation and tubulointerstitial fibrosis leading to end-stage renal disease. Transient receptor potential canonical 6 (TRPC6) channel inhibition mitigates tubular injury and renal fibrosis in murine models of unilateral ureteral obstruction (UUO) and 2-month chronic post-ischemia-reperfusion injury (2m post-I/R). Through integrated analysis of single-cell-RNA-sequencing (scRNA-Seq) data from UUO mice treated with the selective TRPC6 inhibitor SH045, here the renoprotective cell composition and cell type-specific transcriptional programs are defined. We explored translational aspects by conducting an in-depth scRNA-Seq analysis of kidney samples from patients with CKD. These results reveal global transcriptional shifts with a dAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Single-Cell RNA Sequencing Delineates Renal Anti-Fibrotic Mechanisms Mediated by TRPC6 Inhibition.PMC12412467GO766/26‐1SFB1365GO766/25‐1GO766/22-2NSFCGO766/25-1GO766/22‐2GO766/26-1GO766/18-2GO766/18‐282070718FOVB-2023-1223ZR145100001GY220124PJD088Zheng ZLiu JSchaefer MEndlich NKruegel UOswald MSTsvetkov DSimm SGerhardt HCheng GZhai QXu YGollasch MfalseSingle-Cell RNA Sequencing Delineates Renal Anti-Fibrotic Mechanisms Mediated by TRPC6 Inhibition.Chronic kidney disease (CKD) is characterized by persistent inflammation and tubulointerstitial fibrosis leading to end-stage renal disease. Transient receptor potential canonical 6 (TRPC6) channel inhibition mitigates tubular injury and renal fibrosis in murine models of unilateral ureteral obstruction (UUO) and 2-month chronic post-ischemia-reperfusion injury (2m post-I/R). Through integrated analysis of single-cell-RNA-sequencing (scRNA-Seq) data from UUO mice treated with the selective TRPC6 inhibitor SH045, here the renoprotective cell composition and cell type-specific transcriptional programs are defined. We explored translational aspects by conducting an in-depth scRNA-Seq analysis of kidney samples from patients with CKD. These results reveal global transcriptional shifts with a d2025-01-01T00:00:00Z2025 Sep2026-05-29T19:42:26.584Z2026-04-08T05:49:48.859ZS-EPMC124124674052524610.1002/advs.202501175