{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song H"],"funding":["Key Research and Development Program of Heilongjiang Province","Nn10 project at Harbin Medical University Cancer Hospital","Talent Support Program of Harbin Medical University","Heilongjiang Provincial Department of Human Resources and Social Security","Key Project of Heilongjiang Province Outstanding Young Teachers Basic Research","National Natural Science Foundation of China","Heilongjiang Provincial Postdoctoral Science Foundation","\"Young Marshal Unveiling\" Project of Harbin Medical University","Chun-Yan Innovation Team Program from Heilongjiang Provincial Department of Science and Technology","Harbin Medical University Cancer Hospital Haiyan Foundation","National Key Research and Development Program of China","Natural Science Foundation of Heilongjiang Province","Hong Kong scholar project"],"pagination":["e00216"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12412502"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(33)"],"pubmed_abstract":["Penile squamous cell carcinoma (PSCC) is a highly aggressive malignancy without effective treatment due to limited knowledge of its development and tumor microenvironment (TME). In this study, single-nucleus RNA sequencing (snRNA-seq) and high-resolution spatial transcriptomics are employed to comprehensively investigate the development trajectories and the TME. The results revealed that PSCC cells mimicked the differentiation and tissue organization of normal penile epithelium, independent of the human papillomavirus (HPV) infection status. Notably, a spatial subtype, Tum_1, appeared at early stage of tumor differentiation and in tumor-normal boundary regions. This subtype exhibited enhanced basal-like and stemness features and showed high LAMC2 expression, which activated laminin-integri"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Single-Cell and Spatial Transcriptomic Profiling of Penile Squamous Cell Carcinoma Reveals Dynamics of Tumor Differentiation and Immune Microenvironment."],"pmcid":["PMC12412502"],"funding_grant_id":["JJYQ2024-03","2022ZXJ03C07","YQ2024H023","LBH-Z22030","JJZD2024-05","Nn102024-01","YQJH2023034","82302266","JJZD2024-24","82402416","2022YFA1103400","XJ2024052","LBH‐Z22030","2022ZX06004","2021M693828","31021240077","31021240027","CYQN24038","2021YFB3801000","82171999"],"pubmed_authors":["Wu L","Ding D","Shi Q","Hu X","Wang D","Wang J","Su H","Zhao Y","Zhang Q","Guo P","Zhang P","Liu W","Qiu Y","Fan F","Xie G","Liu Z","Deng Z","Chen Y","Wang Z","Wen F","Li G","Li H","Li J","Li C","Tong Z","Nawroth R","Song H","Yin Z","Teng L","Li Y","Yang Z","Zhang Z","Dai P","Hou D","Xu T","Xu W","Meng H","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell and Spatial Transcriptomic Profiling of Penile Squamous Cell Carcinoma Reveals Dynamics of Tumor Differentiation and Immune Microenvironment.","description":"Penile squamous cell carcinoma (PSCC) is a highly aggressive malignancy without effective treatment due to limited knowledge of its development and tumor microenvironment (TME). In this study, single-nucleus RNA sequencing (snRNA-seq) and high-resolution spatial transcriptomics are employed to comprehensively investigate the development trajectories and the TME. The results revealed that PSCC cells mimicked the differentiation and tissue organization of normal penile epithelium, independent of the human papillomavirus (HPV) infection status. Notably, a spatial subtype, Tum_1, appeared at early stage of tumor differentiation and in tumor-normal boundary regions. This subtype exhibited enhanced basal-like and stemness features and showed high LAMC2 expression, which activated laminin-integri","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-29T21:49:32.639Z","creation":"2026-04-08T06:07:33.368Z"},"accession":"S-EPMC12412502","cross_references":{"pubmed":["40470730"],"doi":["10.1002/advs.202500216"]}}