{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ren L"],"funding":["Shanghai Municipal Health Commission Collaborative Innovation Cluster Project","Fundamental Research Funds for the Central Universities","National Natural Science Foundation of China"],"pagination":["e17045"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12412581"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(33)"],"pubmed_abstract":["Diabetes is a prevalent metabolic disorder, and its associated microvascular dysfunction plays a key role in the pathogenesis of complications such as retinopathy, nephropathy, and peripheral vascular disease. However, the mechanism governing metabolic regulation and functional heterogeneity of endothelial dysfunction remains incompletely understood. This study investigates the role of metabolic stress-induced activation of choline kinase α (CHKA) in endothelial cell (EC) subpopulations, contributing to diabetes-induced microvascular dysfunction. Using single-cell RNA sequencing (scRNA-seq), three distinct EC subclusters are identified within retinal vessels. Among them, one subcluster characterized by elevated CHKA expression is associated with enhanced angiogenic activity. CHKA silencing"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Metabolic Stress-Induced Choline Kinase α (CHKA) Activation in Endothelial Subpopulation Contributes to Diabetes-Associated Microvascular Dysfunction."],"pmcid":["PMC12412581"],"funding_grant_id":["82225013","2024CXJQ02","YG2025ZD04"],"pubmed_authors":["Li X","Huang C","Ren L","Yao M","Jiang Q","Jiang C","Zhang L","Mu W","Chen X","Yan B","Bai Y","Ma F"],"additional_accession":[]},"is_claimable":false,"name":"Metabolic Stress-Induced Choline Kinase α (CHKA) Activation in Endothelial Subpopulation Contributes to Diabetes-Associated Microvascular Dysfunction.","description":"Diabetes is a prevalent metabolic disorder, and its associated microvascular dysfunction plays a key role in the pathogenesis of complications such as retinopathy, nephropathy, and peripheral vascular disease. However, the mechanism governing metabolic regulation and functional heterogeneity of endothelial dysfunction remains incompletely understood. This study investigates the role of metabolic stress-induced activation of choline kinase α (CHKA) in endothelial cell (EC) subpopulations, contributing to diabetes-induced microvascular dysfunction. Using single-cell RNA sequencing (scRNA-seq), three distinct EC subclusters are identified within retinal vessels. Among them, one subcluster characterized by elevated CHKA expression is associated with enhanced angiogenic activity. CHKA silencing","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-01T23:25:47.2Z","creation":"2026-05-23T03:07:45.918Z"},"accession":"S-EPMC12412581","cross_references":{"pubmed":["40548950"],"doi":["10.1002/advs.202417045"]}}