{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han X"],"funding":["Chongqing Medical University","National key R&D Program of China","National Natural Science Foundation of China"],"pagination":["gkaf849"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12412784"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(17)"],"pubmed_abstract":["Local pH variations play a pivotal role in numerous critical biological processes. However, achieving the tunability and selectivity of pH detection remains a challenge. Here, we present a DNA-based strategy that enables programmable and selective pH responses, which is termed shadow-strand hybridization-actuated displacement engineering (SHADE). The tunability of pH responses is accomplished via rational manipulations of shadow strands derived from the i-motif-forming sequence. Shadow strands, which can be considered the \"nucleic acid molecular chaperones,\" assist in the folding of i-motif structure under acidic conditions via toehold-mediated strand displacement reactions (TMSDR). The response to alkaline conditions is achieved through a hairpin shadow (HS) containing A+-C pairs in stem."],"journal":["Nucleic acids research"],"pubmed_title":["Rational design of tunable pH switches through shadow-strand hybridization-actuated displacement engineering."],"pmcid":["PMC12412784"],"funding_grant_id":["82172369","BJRC202505","2022YFC2603800"],"pubmed_authors":["Liu X","Jiang J","Yang G","Xie G","Dong X","Guo Y","Zhang Y","Han X","Lin X","Wang W","Zhang L","Yu H"],"additional_accession":[]},"is_claimable":false,"name":"Rational design of tunable pH switches through shadow-strand hybridization-actuated displacement engineering.","description":"Local pH variations play a pivotal role in numerous critical biological processes. However, achieving the tunability and selectivity of pH detection remains a challenge. Here, we present a DNA-based strategy that enables programmable and selective pH responses, which is termed shadow-strand hybridization-actuated displacement engineering (SHADE). The tunability of pH responses is accomplished via rational manipulations of shadow strands derived from the i-motif-forming sequence. Shadow strands, which can be considered the \"nucleic acid molecular chaperones,\" assist in the folding of i-motif structure under acidic conditions via toehold-mediated strand displacement reactions (TMSDR). The response to alkaline conditions is achieved through a hairpin shadow (HS) containing A+-C pairs in stem.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-01T13:58:06.223Z","creation":"2026-04-08T13:18:57.533Z"},"accession":"S-EPMC12412784","cross_references":{"pubmed":["40923757"],"doi":["10.1093/nar/gkaf849"]}}