{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shepherd RA"],"funding":["National Science Foundation Graduate Research Fellowship Program","Division of Integrative Organismal Systems","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["18867-18872"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12413305"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["97(35)"],"pubmed_abstract":["Mass spectrometry imaging (MSI) is a powerful tool for monitoring the spatial distributions of microbial metabolites directly from culture. MSI can identify secretion and retention patterns for microbial metabolites, allowing for the assessment of chemical communication within complex microbial communities. Microbial imaging via matrix-assisted laser desorption/ionization (MALDI) MSI remains challenging due to high sample complexity and heterogeneity associated with the required sample preparation, making annotation of molecules by MS<sup>1</sup> alone challenging. The implementation of trapped ion mobility spectrometry (TIMS) has increased the dimensionality of MALDI-MSI experiments, allowing for the resolution of isomers and isobars, and can increase sensitivity of metabolite detection w"],"journal":["Analytical chemistry"],"pubmed_title":["MALDI-TIMS-MS&lt;sup&gt;2&lt;/sup&gt; Imaging and Annotation of Natural Products in Fungal-Bacterial Coculture."],"pmcid":["PMC12413305"],"funding_grant_id":["2220510","R21 GM148870","R21GM148870"],"pubmed_authors":["Sanchez LM","Shepherd RA","Luu GT"],"additional_accession":[]},"is_claimable":false,"name":"MALDI-TIMS-MS&lt;sup&gt;2&lt;/sup&gt; Imaging and Annotation of Natural Products in Fungal-Bacterial Coculture.","description":"Mass spectrometry imaging (MSI) is a powerful tool for monitoring the spatial distributions of microbial metabolites directly from culture. MSI can identify secretion and retention patterns for microbial metabolites, allowing for the assessment of chemical communication within complex microbial communities. Microbial imaging via matrix-assisted laser desorption/ionization (MALDI) MSI remains challenging due to high sample complexity and heterogeneity associated with the required sample preparation, making annotation of molecules by MS<sup>1</sup> alone challenging. The implementation of trapped ion mobility spectrometry (TIMS) has increased the dimensionality of MALDI-MSI experiments, allowing for the resolution of isomers and isobars, and can increase sensitivity of metabolite detection w","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-02T22:37:06.471Z","creation":"2026-05-28T03:07:01.255Z"},"accession":"S-EPMC12413305","cross_references":{"pubmed":["40864852"],"doi":["10.1021/acs.analchem.5c02787"]}}