biostudies-literatureUnknown15Phetkong C<h4>Introduction</h4>Hepatocellular carcinoma (HCC) remains a major cause of cancer mortality, and effective therapeutic options are limited. MicroRNA‑372‑3p (miR‑372‑3p) has been implicated in HCC, yet its exact role is unclear.<h4>Methods</h4>We established miR‑372‑3p‑overexpressing HCC cell lines (HepG2, SNU‑449, JHH‑4) via lentiviral transduction. Malignant phenotypes were assessed with MTT, transwell migration/invasion, and colony‑formation assays. Transcriptomic changes were analyzed by RNA‑sequencing followed by Gene Set Enrichment Analysis. Lipid metabolism was examined using BODIPY/Oil Red O staining, triglyceride quantification, FAOBlue assays, and organelle colocalization imaging. Candidate targets of miR‑372‑3p were computationally predicted and validated by dual‑luciferase repBioImpacts : BI31075https://www.ebi.ac.uk/biostudies/studies/S-EPMC12413982biostudies-literatureMicroRNA-372-3p impairs fatty acid metabolism in hepatocellular carcinoma cells by targeting <i>CPT1A</i> and <i>ACSL4</i>.PMC12413982Boonto TPhetkong CThamjamrassri PTangkijvanich PAriyachet CfalseMicroRNA-372-3p impairs fatty acid metabolism in hepatocellular carcinoma cells by targeting <i>CPT1A</i> and <i>ACSL4</i>.<h4>Introduction</h4>Hepatocellular carcinoma (HCC) remains a major cause of cancer mortality, and effective therapeutic options are limited. MicroRNA‑372‑3p (miR‑372‑3p) has been implicated in HCC, yet its exact role is unclear.<h4>Methods</h4>We established miR‑372‑3p‑overexpressing HCC cell lines (HepG2, SNU‑449, JHH‑4) via lentiviral transduction. Malignant phenotypes were assessed with MTT, transwell migration/invasion, and colony‑formation assays. Transcriptomic changes were analyzed by RNA‑sequencing followed by Gene Set Enrichment Analysis. Lipid metabolism was examined using BODIPY/Oil Red O staining, triglyceride quantification, FAOBlue assays, and organelle colocalization imaging. Candidate targets of miR‑372‑3p were computationally predicted and validated by dual‑luciferase rep2025-01-01T00:00:00Z20252026-06-02T22:37:03.656Z2026-05-28T03:07:00.328ZS-EPMC124139824092295110.34172/bi.31075