biostudies-literatureUnknown100Yusta BCIHR<h4>Objective</h4>Glucagon-like peptide-1 (GLP-1) reduces systemic and gut inflammation. Here we assessed whether gain or loss of GLP-1 receptor (GLP-1R) signaling modifies the extent of gut injury and inflammation in experimental murine acute graft vs. host disease (aGvHD).<h4>Methods</h4>Allogeneic hematopoietic cell transplantation (HCT) was performed using bone marrow and splenocytes from BALB/c donors to induce aGvHD in C57BL/6 recipients or vice versa. Chimerism was determined by flow cytometry analysis of immune cell compartments. Inflammation was assessed by histological scoring of gut mucosal damage and by measuring circulating cytokine levels. qPCR was used to quantify gene expression in small intestine immune cells and tissues. The gut microbiome was assessed by 16S rRNA sequencMolecular metabolism102235https://www.ebi.ac.uk/biostudies/studies/S-EPMC12414834biostudies-literatureGLP-1R signaling does not modify the severity of experimental graft versus host disease.PMC12414834Bang KAWong CKDrucker DJYusta BKoehler JABaggio LLMatthews DfalseGLP-1R signaling does not modify the severity of experimental graft versus host disease.<h4>Objective</h4>Glucagon-like peptide-1 (GLP-1) reduces systemic and gut inflammation. Here we assessed whether gain or loss of GLP-1 receptor (GLP-1R) signaling modifies the extent of gut injury and inflammation in experimental murine acute graft vs. host disease (aGvHD).<h4>Methods</h4>Allogeneic hematopoietic cell transplantation (HCT) was performed using bone marrow and splenocytes from BALB/c donors to induce aGvHD in C57BL/6 recipients or vice versa. Chimerism was determined by flow cytometry analysis of immune cell compartments. Inflammation was assessed by histological scoring of gut mucosal damage and by measuring circulating cytokine levels. qPCR was used to quantify gene expression in small intestine immune cells and tissues. The gut microbiome was assessed by 16S rRNA sequenc2025-01-01T00:00:00Z2025 Oct2026-06-02T02:24:55.49Z2026-04-13T03:11:37.614ZS-EPMC124148344084607510.1016/j.molmet.2025.102235