biostudies-literatureUnknown27Bakker NAM<h4>Background</h4>Breast cancer is a systemic disease, yet the impact of tumor molecular subtype and disease stage on the systemic immune landscape remains poorly understood. In this study, we comprehensively analyzed the systemic immune landscape in a large cohort of breast cancer patients, encompassing all molecular subtypes and disease stages, alongside a control group of healthy donors.<h4>Materials and methods</h4>Using multi-parameter flow cytometry, we assessed the abundance, phenotype, and activation status of diverse innate and adaptive immune cell populations across peripheral blood samples from 355 breast cancer patients and 65 healthy donors. Analyzing all blood samples immediately after collection enabled analysis of often overlooked, but highly abundant granulocyte populatioImmuno-oncology technology101065https://www.ebi.ac.uk/biostudies/studies/S-EPMC12415084biostudies-literatureComprehensive analysis of the systemic immune landscape across breast cancer subtypes and disease stages.PMC12415084Kok MDuijst MBakker NAMLips EHde Visser KEVoorthuis RKlaver CNederlof IChampanhet EOosterkamp HMGielen RCAMde Graaf MLiefaard MCGarner HGeurts VCMfalseComprehensive analysis of the systemic immune landscape across breast cancer subtypes and disease stages.<h4>Background</h4>Breast cancer is a systemic disease, yet the impact of tumor molecular subtype and disease stage on the systemic immune landscape remains poorly understood. In this study, we comprehensively analyzed the systemic immune landscape in a large cohort of breast cancer patients, encompassing all molecular subtypes and disease stages, alongside a control group of healthy donors.<h4>Materials and methods</h4>Using multi-parameter flow cytometry, we assessed the abundance, phenotype, and activation status of diverse innate and adaptive immune cell populations across peripheral blood samples from 355 breast cancer patients and 65 healthy donors. Analyzing all blood samples immediately after collection enabled analysis of often overlooked, but highly abundant granulocyte populatio2025-01-01T00:00:00Z2025 Sep2026-06-01T16:10:34.068Z2026-04-08T13:59:14.824ZS-EPMC124150844092717910.1016/j.iotech.2025.101065