{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang F"],"funding":["Research on the Efficient Separation of Bull Y-Sperm Using Resiquimod and Polyvinylpyrrolidone","The Selection of Differential Proteins in the Cervical Mucus of Cows Producing Offspring of a Single Gender Continuously","Graduate Student Innovation Project"],"pagination":["1881-1893"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12415464"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(9)"],"pubmed_abstract":["<h4>Objective</h4>The X/Y sperm separation technique plays a crucial role in gender control. The objective of this experiment is to investigate the effect of polyvinylpyrrolidone (PVP) concentration (A: 0%, B: 1%, C: 3%, D: 5%, E: 7%, vol/vol) on Y sperm sorting efficiency, based on the specific binding of Resiquimod (R848) to toll-like receptor (TLR)7/8 receptors on the tail of X sperm.<h4>Methods</h4>The different concentrations of PVP were added to the R848 sperm sorting solution to facilitate the separation of Y sperm. Subsequently, the isolated sperm were subjected to quantification and motility assessment using computer-assisted semen analysis system. The X/Y sperm ratio is then analyzed by flow cytometry. The sorted sperm were evaluated for acrosomal and plasma membrane integrity. T"],"journal":["Animal bioscience"],"pubmed_title":["Polyvinylpyrrolidone can effectively improve the efficiency of resiquimod in sorting bovine Y sperm."],"pmcid":["PMC12415464"],"funding_grant_id":["TDGRI2024033","TDBSCX20248","TDBSCX202417","TDZKZD202404","TDBSCX202212"],"pubmed_authors":["Huang F","Gao QH","Wang JR","Fang D","Niu P","Suo JJ","Qu HM","Cheng H","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"Polyvinylpyrrolidone can effectively improve the efficiency of resiquimod in sorting bovine Y sperm.","description":"<h4>Objective</h4>The X/Y sperm separation technique plays a crucial role in gender control. The objective of this experiment is to investigate the effect of polyvinylpyrrolidone (PVP) concentration (A: 0%, B: 1%, C: 3%, D: 5%, E: 7%, vol/vol) on Y sperm sorting efficiency, based on the specific binding of Resiquimod (R848) to toll-like receptor (TLR)7/8 receptors on the tail of X sperm.<h4>Methods</h4>The different concentrations of PVP were added to the R848 sperm sorting solution to facilitate the separation of Y sperm. Subsequently, the isolated sperm were subjected to quantification and motility assessment using computer-assisted semen analysis system. The X/Y sperm ratio is then analyzed by flow cytometry. The sorted sperm were evaluated for acrosomal and plasma membrane integrity. T","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-02T02:25:10.704Z","creation":"2026-04-13T03:11:42.363Z"},"accession":"S-EPMC12415464","cross_references":{"pubmed":["40211851"],"doi":["10.5713/ab.24.0738"]}}