biostudies-literatureHuang FResearch on the Efficient Separation of Bull Y-Sperm Using Resiquimod and PolyvinylpyrrolidoneThe Selection of Differential Proteins in the Cervical Mucus of Cows Producing Offspring of a Single Gender ContinuouslyGraduate Student Innovation Project1881-1893https://www.ebi.ac.uk/biostudies/studies/S-EPMC12415464biostudies-literatureUnknown38(9)<h4>Objective</h4>The X/Y sperm separation technique plays a crucial role in gender control. The objective of this experiment is to investigate the effect of polyvinylpyrrolidone (PVP) concentration (A: 0%, B: 1%, C: 3%, D: 5%, E: 7%, vol/vol) on Y sperm sorting efficiency, based on the specific binding of Resiquimod (R848) to toll-like receptor (TLR)7/8 receptors on the tail of X sperm.<h4>Methods</h4>The different concentrations of PVP were added to the R848 sperm sorting solution to facilitate the separation of Y sperm. Subsequently, the isolated sperm were subjected to quantification and motility assessment using computer-assisted semen analysis system. The X/Y sperm ratio is then analyzed by flow cytometry. The sorted sperm were evaluated for acrosomal and plasma membrane integrity. TAnimal biosciencePolyvinylpyrrolidone can effectively improve the efficiency of resiquimod in sorting bovine Y sperm.PMC12415464TDGRI2024033TDBSCX20248TDBSCX202417TDZKZD202404TDBSCX202212Huang FGao QHWang JRFang DNiu PSuo JJQu HMCheng HWang JfalsePolyvinylpyrrolidone can effectively improve the efficiency of resiquimod in sorting bovine Y sperm.<h4>Objective</h4>The X/Y sperm separation technique plays a crucial role in gender control. The objective of this experiment is to investigate the effect of polyvinylpyrrolidone (PVP) concentration (A: 0%, B: 1%, C: 3%, D: 5%, E: 7%, vol/vol) on Y sperm sorting efficiency, based on the specific binding of Resiquimod (R848) to toll-like receptor (TLR)7/8 receptors on the tail of X sperm.<h4>Methods</h4>The different concentrations of PVP were added to the R848 sperm sorting solution to facilitate the separation of Y sperm. Subsequently, the isolated sperm were subjected to quantification and motility assessment using computer-assisted semen analysis system. The X/Y sperm ratio is then analyzed by flow cytometry. The sorted sperm were evaluated for acrosomal and plasma membrane integrity. T2025-01-01T00:00:00Z2025 Sep2026-06-02T02:25:10.704Z2026-04-13T03:11:42.363ZS-EPMC124154644021185110.5713/ab.24.0738