{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(16)"],"submitter":["Wessels U"],"funding":["Roche Diagnostics"],"pubmed_abstract":["<h4>Aim</h4>To confirm the observation of IgM interference in the anti-adeno-associated virus (AAV) IgG immune complex (IC) assay format and to verify that IgM-specific digestion can improve anti-AAV IgG detection in IC assays.<h4>Methods</h4>Treatment-emergent anti-AAV2 and anti-AAV9 IgG signals were measured in IC assays with and without IgM-specific digestion. Anti-AAV2 and anti-AAV9 IgM signals were measured in parallel.<h4>Results</h4>IgM digestion increased anti-AAV2 and anti-AAV9 IgG signals when anti-AAV2 or anti-AAV9 IgM were present in the matrix.<h4>Conclusions</h4>Co-existing anti-AAV IgM cause interference in the anti-AAV IC assay format. Selective IgM digestion improves the detection of anti-AAV IgG in the IC assay."],"journal":["Bioanalysis"],"pagination":["1011-1016"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12416191"],"repository":["biostudies-literature"],"pubmed_title":["Selective IgM digestion improves anti-AAV IgG detection in the immune complex assay format."],"pmcid":["PMC12416191"],"pubmed_authors":["Wessels U","Stubenrauch KG","Pohler A","Ros F"],"additional_accession":[]},"is_claimable":false,"name":"Selective IgM digestion improves anti-AAV IgG detection in the immune complex assay format.","description":"<h4>Aim</h4>To confirm the observation of IgM interference in the anti-adeno-associated virus (AAV) IgG immune complex (IC) assay format and to verify that IgM-specific digestion can improve anti-AAV IgG detection in IC assays.<h4>Methods</h4>Treatment-emergent anti-AAV2 and anti-AAV9 IgG signals were measured in IC assays with and without IgM-specific digestion. Anti-AAV2 and anti-AAV9 IgM signals were measured in parallel.<h4>Results</h4>IgM digestion increased anti-AAV2 and anti-AAV9 IgG signals when anti-AAV2 or anti-AAV9 IgM were present in the matrix.<h4>Conclusions</h4>Co-existing anti-AAV IgM cause interference in the anti-AAV IC assay format. Selective IgM digestion improves the detection of anti-AAV IgG in the IC assay.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-02T02:45:00.858Z","creation":"2026-04-13T03:12:53.918Z"},"accession":"S-EPMC12416191","cross_references":{"pubmed":["40899655"],"doi":["10.1080/17576180.2025.2548191"]}}