biostudies-literatureUnknown17(16)Wessels URoche Diagnostics<h4>Aim</h4>To confirm the observation of IgM interference in the anti-adeno-associated virus (AAV) IgG immune complex (IC) assay format and to verify that IgM-specific digestion can improve anti-AAV IgG detection in IC assays.<h4>Methods</h4>Treatment-emergent anti-AAV2 and anti-AAV9 IgG signals were measured in IC assays with and without IgM-specific digestion. Anti-AAV2 and anti-AAV9 IgM signals were measured in parallel.<h4>Results</h4>IgM digestion increased anti-AAV2 and anti-AAV9 IgG signals when anti-AAV2 or anti-AAV9 IgM were present in the matrix.<h4>Conclusions</h4>Co-existing anti-AAV IgM cause interference in the anti-AAV IC assay format. Selective IgM digestion improves the detection of anti-AAV IgG in the IC assay.Bioanalysis1011-1016https://www.ebi.ac.uk/biostudies/studies/S-EPMC12416191biostudies-literatureSelective IgM digestion improves anti-AAV IgG detection in the immune complex assay format.PMC12416191Wessels UStubenrauch KGPohler ARos FfalseSelective IgM digestion improves anti-AAV IgG detection in the immune complex assay format.<h4>Aim</h4>To confirm the observation of IgM interference in the anti-adeno-associated virus (AAV) IgG immune complex (IC) assay format and to verify that IgM-specific digestion can improve anti-AAV IgG detection in IC assays.<h4>Methods</h4>Treatment-emergent anti-AAV2 and anti-AAV9 IgG signals were measured in IC assays with and without IgM-specific digestion. Anti-AAV2 and anti-AAV9 IgM signals were measured in parallel.<h4>Results</h4>IgM digestion increased anti-AAV2 and anti-AAV9 IgG signals when anti-AAV2 or anti-AAV9 IgM were present in the matrix.<h4>Conclusions</h4>Co-existing anti-AAV IgM cause interference in the anti-AAV IC assay format. Selective IgM digestion improves the detection of anti-AAV IgG in the IC assay.2025-01-01T00:00:00Z2025 Aug2026-06-02T02:45:00.858Z2026-04-13T03:12:53.918ZS-EPMC124161914089965510.1080/17576180.2025.2548191