biostudies-literatureUnknown73(8)Deivarajan HR<h4>Purpose</h4>This study introduces and evaluates the single-tube rapid identification of mycoses using CRISPR (ST-RID-MyC) assay. This novel diagnostic tool combines recombinase polymerase amplification (RPA) with CRISPR/Cas12a for the rapid and precise diagnosis of fungal keratitis (FK).<h4>Design</h4>Prospective cross-sectional study.<h4>Methods</h4>Corneal scrapings from 61 patients with suspected microbial keratitis were collected at the Cornea Department of a Tertiary Eye Care Center. The study assessed the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the ST-RID-MyC assay. Additional measures included concordance rates with traditional diagnostic methods and the time to diagnosis.<h4>Results</h4>The ST-RID-MyC assay exhibited a sIndian journal of ophthalmology1208-1212https://www.ebi.ac.uk/biostudies/studies/S-EPMC12416573biostudies-literatureRapid one-tube RPA-coupled CRISPR/Cas12a-based RID-MyC assay for the diagnosis of fungal keratitis.PMC12416573Elamurugan VKuppamuthu DDeivarajan HRPandian JSenthilkumar KPrajna LPrajna VNSekar HVVenugopal ANarendran SfalseRapid one-tube RPA-coupled CRISPR/Cas12a-based RID-MyC assay for the diagnosis of fungal keratitis.<h4>Purpose</h4>This study introduces and evaluates the single-tube rapid identification of mycoses using CRISPR (ST-RID-MyC) assay. This novel diagnostic tool combines recombinase polymerase amplification (RPA) with CRISPR/Cas12a for the rapid and precise diagnosis of fungal keratitis (FK).<h4>Design</h4>Prospective cross-sectional study.<h4>Methods</h4>Corneal scrapings from 61 patients with suspected microbial keratitis were collected at the Cornea Department of a Tertiary Eye Care Center. The study assessed the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the ST-RID-MyC assay. Additional measures included concordance rates with traditional diagnostic methods and the time to diagnosis.<h4>Results</h4>The ST-RID-MyC assay exhibited a s2025-01-01T00:00:00Z2025 Aug2026-04-13T03:30:17.571Z2026-04-13T03:12:46.519ZS-EPMC124165734071972610.4103/IJO.IJO_1613_24