{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ghonyan S"],"funding":["Higher Education and Science Committee, The RA Ministry of Education, Science, Culture and Sports"],"pagination":["e0331783"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12416653"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(9)"],"pubmed_abstract":["The short lifespan of polymorphonuclear neutrophils (PMNs) in vitro poses challenges, as their limited viability restricts functional assays and experimental manipulations. The HL-60 cell line serves as a valuable model for neutrophil-like differentiation, yet the functional relevance of ATRA- and DMSO-induced differentiation remains incompletely understood. In the present study, we aimed to characterize the differentiation potential of all-trans retinoic acid (ATRA) and dimethyl sulfoxide (DMSO) on HL-60 cells and compare their functionality with primary PMNs. Besides that, we performed profound immunophenotypes of the cells with multicolor cytometry, and evaluated their antitumor capabilities. Our findings indicate that both differentiation conditions yield cells resembling immature neut"],"journal":["PloS one"],"pubmed_title":["Unraveling the functional landscape of ATRA- and DMSO-differentiated HL-60 cells."],"pmcid":["PMC12416653"],"funding_grant_id":["SCS 24AA-1F007","SCS 21AG-1F072"],"pubmed_authors":["Manukyan G","Martirosyan A","Margaryan S","Khachatryan Z","Poghosyan D","Ghonyan S","Avetisyan A"],"additional_accession":[]},"is_claimable":false,"name":"Unraveling the functional landscape of ATRA- and DMSO-differentiated HL-60 cells.","description":"The short lifespan of polymorphonuclear neutrophils (PMNs) in vitro poses challenges, as their limited viability restricts functional assays and experimental manipulations. The HL-60 cell line serves as a valuable model for neutrophil-like differentiation, yet the functional relevance of ATRA- and DMSO-induced differentiation remains incompletely understood. In the present study, we aimed to characterize the differentiation potential of all-trans retinoic acid (ATRA) and dimethyl sulfoxide (DMSO) on HL-60 cells and compare their functionality with primary PMNs. Besides that, we performed profound immunophenotypes of the cells with multicolor cytometry, and evaluated their antitumor capabilities. Our findings indicate that both differentiation conditions yield cells resembling immature neut","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-02T02:27:10.783Z","creation":"2026-04-13T03:11:58.968Z"},"accession":"S-EPMC12416653","cross_references":{"pubmed":["40920704"],"doi":["10.1371/journal.pone.0331783"]}}