biostudies-literatureUnknown15Li G<h4>Background</h4>Gliomas, particularly glioblastoma, are aggressive brain tumors with poor prognosis and unmet therapeutic needs. Structural maintenance of chromosomes 4 (SMC4), a core component of the condensin complex, is dysregulated in multiple cancers, but its role in glioma metabolism and metastasis remains unclear.<h4>Methods</h4>Using integrated multi-omics analyses of glioma datasets, we assessed SMC4 expression and its correlation with clinical outcomes. Functional studies in U-251MG and LN229 glioma cells including CCK-8, EdU, cell cycle, Transwell, and wound-healing assays were combined with subcutaneous xenograft and tail-vein metastasis mouse models to evaluate SMC4's effects on proliferation, migration, invasion, and metastasis. ECAR/OCR and rescue experiments validated SMFrontiers in oncology1624370https://www.ebi.ac.uk/biostudies/studies/S-EPMC12417569biostudies-literatureNFIA-dependent upregulation of SMC4 promotes metastasis and metabolic reprogramming in glioma.PMC12417569Li GLi XQian ZLiu ZHe YZhao YSong ZfalseNFIA-dependent upregulation of SMC4 promotes metastasis and metabolic reprogramming in glioma.<h4>Background</h4>Gliomas, particularly glioblastoma, are aggressive brain tumors with poor prognosis and unmet therapeutic needs. Structural maintenance of chromosomes 4 (SMC4), a core component of the condensin complex, is dysregulated in multiple cancers, but its role in glioma metabolism and metastasis remains unclear.<h4>Methods</h4>Using integrated multi-omics analyses of glioma datasets, we assessed SMC4 expression and its correlation with clinical outcomes. Functional studies in U-251MG and LN229 glioma cells including CCK-8, EdU, cell cycle, Transwell, and wound-healing assays were combined with subcutaneous xenograft and tail-vein metastasis mouse models to evaluate SMC4's effects on proliferation, migration, invasion, and metastasis. ECAR/OCR and rescue experiments validated SM2025-01-01T00:00:00Z20252026-05-26T11:03:43.02Z2026-05-24T03:07:30.636ZS-EPMC124175694093389410.3389/fonc.2025.1624370