biostudies-literatureUnknown9(9)Tucci MBiological methanation is increasingly considered for biogas upgrading. Here, the supplementation of conductive magnetite (Fe₃O₄) nanoparticles is investigated as a strategy to enhance H₂-driven biomethanation in a mixed hydrogenotrophic methanogenic community. An enrichment culture, maintained for over 180 days in a fill-and-draw anaerobic bioreactor under H₂/CO₂ feeding, is used to inoculate batch microcosms containing 0, 1.25, and 2.5 gFe L^-1 of magnetite. Magnetite addition resulted in a dose-dependent increase in maximum methane production rates-up to 13-fold compared to controls-and sustained high hydrogen-to-methane conversion yields (78-107%). 16S rRNA gene sequencing reveals that archaeal community composition remaiGlobal challenges (Hoboken, NJ)e00367https://www.ebi.ac.uk/biostudies/studies/S-EPMC12418360biostudies-literatureMagnetite Nanoparticles Enhancing H<sub>2</sub>-Driven Biomethanation in a Mixed Microbial Community.PMC12418360Tucci MIosi FFirrincieli AMatturro BSabangan JICruz Viggi CBertaccini LPiacentini AD'Ugo EAulenta FUccelletti DFazi SfalseMagnetite Nanoparticles Enhancing H<sub>2</sub>-Driven Biomethanation in a Mixed Microbial Community.Biological methanation is increasingly considered for biogas upgrading. Here, the supplementation of conductive magnetite (Fe₃O₄) nanoparticles is investigated as a strategy to enhance H₂-driven biomethanation in a mixed hydrogenotrophic methanogenic community. An enrichment culture, maintained for over 180 days in a fill-and-draw anaerobic bioreactor under H₂/CO₂ feeding, is used to inoculate batch microcosms containing 0, 1.25, and 2.5 gFe L^-1 of magnetite. Magnetite addition resulted in a dose-dependent increase in maximum methane production rates-up to 13-fold compared to controls-and sustained high hydrogen-to-methane conversion yields (78-107%). 16S rRNA gene sequencing reveals that archaeal community composition remai2025-01-01T00:00:00Z2025 Sep2026-06-02T00:19:07.384Z2026-05-24T03:07:57.719ZS-EPMC124183604093336010.1002/gch2.202500367