biostudies-literatureBudde KNIAID NIH HHS102619https://www.ebi.ac.uk/biostudies/studies/S-EPMC12418925biostudies-literatureUnknown152The diagnosis of tuberculosis largely relies on the detection of Mycobacterium tuberculosis (M. tuberculosis) via pathogen-specific DNA or bacterial culture from sputum samples. As the only point-of-care test so far, urinary lipoarabinomannan (LAM) has been endorsed by the World Health Organization for the diagnosis of tuberculosis in people living with HIV. In this study, the electrochemiluminescence LAM research assay (EclLAM) was used to measure LAM in the urine of HIV-sero-negative individuals with pulmonary tuberculosis and to monitor anti-tuberculosis treatment. Urine samples from 18 patients with microbiologically confirmed tuberculosis were analyzed before and after the initiation of anti-tuberculosis therapy and 17 healthy controls via the S4-20/A194-01 antibody pair. The assay idTuberculosis (Edinburgh, Scotland)A novel method for detecting Lipoarabinomannan in urine with the promise of meeting the WHO target product profile for the diagnosis of tuberculosis.PMC12418925R01 AI137527Carballo PSMaurer FPTerhalle EDZIF TB cohort study groupMandalakas AMMuller MUnnewehr MGunther GKohler NRachow AAvsar KHoelscher MSchaub DGoldmann TReimann MDiNardo AKalsdorf BBudde KIbraim ESchuhmann MLange CPopa CSalzer HJFZielinski NRolling THeyckendorf JOlaru IDSpinu VMeiwes LKaufmann SHEfalseA novel method for detecting Lipoarabinomannan in urine with the promise of meeting the WHO target product profile for the diagnosis of tuberculosis.The diagnosis of tuberculosis largely relies on the detection of Mycobacterium tuberculosis (M. tuberculosis) via pathogen-specific DNA or bacterial culture from sputum samples. As the only point-of-care test so far, urinary lipoarabinomannan (LAM) has been endorsed by the World Health Organization for the diagnosis of tuberculosis in people living with HIV. In this study, the electrochemiluminescence LAM research assay (EclLAM) was used to measure LAM in the urine of HIV-sero-negative individuals with pulmonary tuberculosis and to monitor anti-tuberculosis treatment. Urine samples from 18 patients with microbiologically confirmed tuberculosis were analyzed before and after the initiation of anti-tuberculosis therapy and 17 healthy controls via the S4-20/A194-01 antibody pair. The assay id2025-01-01T00:00:00Z2025 May2026-06-02T01:02:49.499Z2026-05-24T03:06:55.756ZS-EPMC124189254004896110.1016/j.tube.2025.102619