biostudies-literatureUnknown121Shu PNational Natural Science Foundation of ChinaThis study pioneers a green, efficient ultrasound-assisted extraction (UAE) technique using a deep eutectic solvent (DES) to isolate polysaccharides from Cercis chinensis bark, overcoming limitations of conventional methods. Through systematic screening of 15 DES systems, DES-11 (L-proline:ethylene glycol, 1:2 M ratio) was identified as optimal, achieving a polysaccharide yield of 64.7 mg/g-3.7-fold higher than water extraction. Extraction conditions were optimized via response surface methodology (RSM) to 39 % water content, 45 °C, 28 min, solid-liquid ratio 1:40 g/mL, and 240 W ultrasonic power. SEM analysis confirmed that DES-11 disrupts plant cell walls, enhancing mass transfer. Kinetic studies revealed the process follows pseudo-first-order kinetics (R² > 0.90) and is primaUltrasonics sonochemistry107535https://www.ebi.ac.uk/biostudies/studies/S-EPMC12418997biostudies-literatureUltrasound-assisted deep eutectic solvent extraction of polysaccharides from Cercis chinensis bark: Optimization, kinetics and antioxidant activities.PMC12418997Wu MFan XHuang JYu FWei XLiu SWang HZhang LMeng YFan SWang NYin HShu PHu DWang XfalseUltrasound-assisted deep eutectic solvent extraction of polysaccharides from Cercis chinensis bark: Optimization, kinetics and antioxidant activities.This study pioneers a green, efficient ultrasound-assisted extraction (UAE) technique using a deep eutectic solvent (DES) to isolate polysaccharides from Cercis chinensis bark, overcoming limitations of conventional methods. Through systematic screening of 15 DES systems, DES-11 (L-proline:ethylene glycol, 1:2 M ratio) was identified as optimal, achieving a polysaccharide yield of 64.7 mg/g-3.7-fold higher than water extraction. Extraction conditions were optimized via response surface methodology (RSM) to 39 % water content, 45 °C, 28 min, solid-liquid ratio 1:40 g/mL, and 240 W ultrasonic power. SEM analysis confirmed that DES-11 disrupts plant cell walls, enhancing mass transfer. Kinetic studies revealed the process follows pseudo-first-order kinetics (R² > 0.90) and is prima2025-01-01T00:00:00Z2025 Aug2026-05-26T11:09:16.809Z2026-05-24T03:07:39.566ZS-EPMC124189974088640910.1016/j.ultsonch.2025.107535