{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chudnovskiy A"],"funding":["NIAID NIH HHS","HSRD VA"],"pagination":["eadq8843"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12419230"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(100)"],"pubmed_abstract":["Dendritic cells (DCs) are uniquely capable of transporting tumor antigens to tumor-draining lymph nodes (tdLNs) and interact with effector T cells in the tumor microenvironment (TME) itself, mediating both natural antitumor immunity and the response to checkpoint blockade immunotherapy. Using LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts)-based single-cell transcriptomics, we identified individual DCs capable of presenting antigen to CD4<sup>+</sup> T cells in both the tdLN and TME. Our findings revealed that DCs with similar hyperactivated transcriptional phenotypes interact with helper T cells both in tumors and in the tdLN and that checkpoint blockade drugs enhance these interactions. These findings show that a relatively small fraction of DCs is responsible"],"journal":["Science immunology"],"pubmed_title":["Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4&lt;sup&gt;+&lt;/sup&gt; T cell response."],"pmcid":["PMC12419230"],"funding_grant_id":["DP1 AI144248","R21 AI163813","R21 AI178396","R01 AI108651","SDP 10-044"],"pubmed_authors":["Victora GD","Sade-Feldman M","Phillips BK","Pae J","Bortolatto J","Pasqual G","Chudnovskiy A","Nakandakari-Higa S","Mesin L","Castro TBR","Lu LF","Hacohen N","Schweitzer LD","Cui A","Lin CH"],"additional_accession":[]},"is_claimable":false,"name":"Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4&lt;sup&gt;+&lt;/sup&gt; T cell response.","description":"Dendritic cells (DCs) are uniquely capable of transporting tumor antigens to tumor-draining lymph nodes (tdLNs) and interact with effector T cells in the tumor microenvironment (TME) itself, mediating both natural antitumor immunity and the response to checkpoint blockade immunotherapy. Using LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts)-based single-cell transcriptomics, we identified individual DCs capable of presenting antigen to CD4<sup>+</sup> T cells in both the tdLN and TME. Our findings revealed that DCs with similar hyperactivated transcriptional phenotypes interact with helper T cells both in tumors and in the tdLN and that checkpoint blockade drugs enhance these interactions. These findings show that a relatively small fraction of DCs is responsible","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-02T17:40:30.374Z","creation":"2026-04-18T03:11:25.656Z"},"accession":"S-EPMC12419230","cross_references":{"pubmed":["39365874"],"doi":["10.1126/sciimmunol.adq8843"]}}