biostudies-literatureUnknown123(5)Almeida JEuropean CommissionBiotechnology and Biological Sciences Research CouncilPlastoglobuli (PG) are plant lipoprotein compartments, present in plastid organelles. They are involved in the formation and/or storage of lipophilic metabolites. FIBRILLINs (FBNs) are one of the main PG-associated proteins and are particularly abundant in carotenoid-enriched chromoplasts found in ripe fruits and flowers. To address the contribution of different FBNs, independently and in combination, to isoprenoid formation and sequestration, a multiplex gene editing approach was undertaken in tomato. This approach generated a suite of single and high-order fbn mutants that were shown to lack transcripts and respective protein products. The major PG-related FBNs in tomato chosen for this study are SlFBN1, SlFBN2a, SlFBN4 and SlFBN7a. When knocked out independently, functional redundancy wThe Plant journal : for cell and molecular biologye70447https://www.ebi.ac.uk/biostudies/studies/S-EPMC12419240biostudies-literatureThe FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism.PMC12419240Fraser PDDrapal MEnfissi EMAPerez-Fons LAlmeida JLiew KfalseThe FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism.Plastoglobuli (PG) are plant lipoprotein compartments, present in plastid organelles. They are involved in the formation and/or storage of lipophilic metabolites. FIBRILLINs (FBNs) are one of the main PG-associated proteins and are particularly abundant in carotenoid-enriched chromoplasts found in ripe fruits and flowers. To address the contribution of different FBNs, independently and in combination, to isoprenoid formation and sequestration, a multiplex gene editing approach was undertaken in tomato. This approach generated a suite of single and high-order fbn mutants that were shown to lack transcripts and respective protein products. The major PG-related FBNs in tomato chosen for this study are SlFBN1, SlFBN2a, SlFBN4 and SlFBN7a. When knocked out independently, functional redundancy w2025-01-01T00:00:00Z2025 Sep2026-05-26T17:00:57Z2026-05-24T03:07:49.449ZS-EPMC124192404092402310.1111/tpj.70447