biostudies-literatureKorber KLERDF Programme Johannes Amos ComeniusImpulseproject University Jenae70459https://www.ebi.ac.uk/biostudies/studies/S-EPMC12419790biostudies-literatureUnknown123(5)Progestogens and androgens are steroids found in a wide range of plants, but little is known about their physiological functions. In this study, we sowed seeds of angiosperms on progestogen- and androgen-containing medium and analysed their morphological effects. We further investigated the effects of progesterone and testosterone on brassinosteroid profiles and gene expression in A. thaliana. Additionally, we examined the effects of progesterone and testosterone on A. thaliana plants overexpressing the steroid 5α-reductase DET2. We found that progestogens and androgens have strong negative effects on root length, especially in Brassicaceae species. In addition, these steroids led to uncoordinated cell growth and increased lateral root formation. We failed to detect an effect on endogenousThe Plant journal : for cell and molecular biologyProgestogens and androgens influence root morphology of angiosperms in a brassinosteroid-independent manner.PMC12419790IP 2022-13CZ.02.01.01/00/22_008/0004581Oktay CBarth EFeistel FKorber KLFurch ACUOppermann HStrnad MOklestkova JPaul SDorfner MMunkert JKlein JfalseProgestogens and androgens influence root morphology of angiosperms in a brassinosteroid-independent manner.Progestogens and androgens are steroids found in a wide range of plants, but little is known about their physiological functions. In this study, we sowed seeds of angiosperms on progestogen- and androgen-containing medium and analysed their morphological effects. We further investigated the effects of progesterone and testosterone on brassinosteroid profiles and gene expression in A. thaliana. Additionally, we examined the effects of progesterone and testosterone on A. thaliana plants overexpressing the steroid 5α-reductase DET2. We found that progestogens and androgens have strong negative effects on root length, especially in Brassicaceae species. In addition, these steroids led to uncoordinated cell growth and increased lateral root formation. We failed to detect an effect on endogenous2025-01-01T00:00:00Z2025 Sep2026-06-02T00:18:54.544Z2026-05-24T03:07:55.342ZS-EPMC124197904092493610.1111/tpj.70459