biostudies-literatureBrodeau CINSPIRE ProgramIHU HealthAge of Toulouse (French National Research Agency (ANR) as part of the France 2030 programProgramme d'Investissements d'Avenir and the Agence Nationale pour la Recherche for the national infrastructure ECELLFranceEUR CARee70153https://www.ebi.ac.uk/biostudies/studies/S-EPMC12419852biostudies-literatureUnknown24(9)Aging leads to a decline in physiological reserves, an increase in age-related diseases, reduced functional ability and a shortened healthspan. While molecular markers of chronological aging exist, their link to general health and intrinsic capacity (IC), a composite measure of physical and mental capacities, remains unclear. This study integrates the WHO's Healthy Aging framework with geroscience to explore fibroblasts as indicators of health. We assessed primary skin fibroblasts from 133 individuals aged 20-96, evaluating their ability to maintain tissue structure, modulate immune responses and regulate metabolism (SIM functions). By combining functional and molecular analyses, we investigated the relationship between fibroblast performance, chronological age and IC. Our results demonstrAging cellIdentification of Functional Cellular Markers Related to Human Health, Frailty and Chronological Age.PMC12419852ANR 23-IAHU-0011ANR-18-EURE-00031901175PIA-ANR-11-INBS-005MP0022856Joly CYart AAder ITanner KTCohen AAEspagnolle Nde Souto Barreto PNakhle JCarriere ABlase VFassy JLu WHTeste OIHU HealthAge/Open Science groupChekroun ACasteilla LPlanat VDray CBrodeau CTertrais MTremblay-Franco MGuyonnet SfalseIdentification of Functional Cellular Markers Related to Human Health, Frailty and Chronological Age.Aging leads to a decline in physiological reserves, an increase in age-related diseases, reduced functional ability and a shortened healthspan. While molecular markers of chronological aging exist, their link to general health and intrinsic capacity (IC), a composite measure of physical and mental capacities, remains unclear. This study integrates the WHO's Healthy Aging framework with geroscience to explore fibroblasts as indicators of health. We assessed primary skin fibroblasts from 133 individuals aged 20-96, evaluating their ability to maintain tissue structure, modulate immune responses and regulate metabolism (SIM functions). By combining functional and molecular analyses, we investigated the relationship between fibroblast performance, chronological age and IC. Our results demonstr2025-01-01T00:00:00Z2025 Sep2026-06-02T17:43:02.883Z2026-05-27T03:07:06.537ZS-EPMC124198524059148410.1111/acel.70153