{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu Z"],"funding":["High-level Talent Research Project of Shihezi University","National Natural Science Foundation of China","Talent Program 'Tianchi Talent (Young Doctor)' in Xinjiang Uygur Autonomous Region (CZ004336)","Major Scientific and Technological Projects of Corps"],"pagination":["e70223"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12420658"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(9)"],"pubmed_abstract":["The Brucella abortus A19 attenuated live vaccine poses potential infection risks during practical applications and interferes with serological diagnostics, thereby affecting quarantine measures and the establishment of disease-free zones. Consequently, this study aimed to reduce its potential virulence, enhance its protective efficacy and differentiate it from wild-type strains by knocking out the immunosuppressive virulence gene btpB in the A19 strain. Using homologous recombination, we successfully obtained the A19ΔBtpB deletion strain. In a mouse model, the A19ΔBtpB strain demonstrated improved safety and significantly increased TLR2 and TLR4 expression levels in splenic lymphocytes, suggesting attenuated immune suppression. The A19ΔBtpB strain induced Brucella-specific IgG antibodies c"],"journal":["Microbial biotechnology"],"pubmed_title":["A BtpB-Deleted Brucella Vaccine Strain Provides Dual Advantages: Enhanced Protection and Diagnostic Differentiation Capability in Mice."],"pmcid":["PMC12420658"],"funding_grant_id":["U1803236","RCZK202456","2017AA003","CZ004336"],"pubmed_authors":["Zhang H","He H","Ma Z","Gao H","Chen C","Yi J","Wu A","Zhang Y","Wang Y","Wang Z","Xu Z","Deng X","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"A BtpB-Deleted Brucella Vaccine Strain Provides Dual Advantages: Enhanced Protection and Diagnostic Differentiation Capability in Mice.","description":"The Brucella abortus A19 attenuated live vaccine poses potential infection risks during practical applications and interferes with serological diagnostics, thereby affecting quarantine measures and the establishment of disease-free zones. Consequently, this study aimed to reduce its potential virulence, enhance its protective efficacy and differentiate it from wild-type strains by knocking out the immunosuppressive virulence gene btpB in the A19 strain. Using homologous recombination, we successfully obtained the A19ΔBtpB deletion strain. In a mouse model, the A19ΔBtpB strain demonstrated improved safety and significantly increased TLR2 and TLR4 expression levels in splenic lymphocytes, suggesting attenuated immune suppression. The A19ΔBtpB strain induced Brucella-specific IgG antibodies c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T05:42:14.956Z","creation":"2026-04-25T03:17:19.272Z"},"accession":"S-EPMC12420658","cross_references":{"pubmed":["40926333"],"doi":["10.1111/1751-7915.70223"]}}