{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(9)"],"submitter":["Sun S"],"pubmed_abstract":["<h4>Background</h4>Improving the efficacy of anti-programmed death 1 (PD-1) monoclonal antibody (mAb) therapy remains a major challenge for cancer immunotherapy in non-small cell lung cancer (NSCLC). Gut microbial metabolites can influence immunotherapy efficacy.<h4>Methods</h4>ELISA was used to compare the serum 5-hydroxyindoleacetic acid (5-HIAA) level in patients with NSCLC. Humanized mice were constructed to observe the effect of 5-HIAA on immunotherapy. RNA-seq and flow cytometry were used to analyze the effect of 5-HIAA on tumor-infiltrating lymphocytes. The effects of phenelzine (Phe) and <i>Akkermansia muciniphila</i> (<i>AKK</i>) on 5-HIAA synthesis, antitumor immunity and immunotherapy efficacy were analyzed. Finally, the synergistic effect of Phe combined with <i>AKK</i> on anti"],"journal":["Journal for immunotherapy of cancer"],"pagination":["e011831"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12421609"],"repository":["biostudies-literature"],"pubmed_title":["Enhancing immunotherapy efficacy in NSCLC through the combined use of phenelzine and &lt;i&gt;Akkermansia muciniphila&lt;/i&gt; to regulate gut microbial metabolite 5-HIAA."],"pmcid":["PMC12421609"],"pubmed_authors":["Zheng Y","Cui K","Li C","Chao K","Yu P","Wei Y","Li L","Wang L","Ding Y","Shen Z","Yang Y","Ji W","Zhu L","Zhang Y","Ge Y","Sun S","Sethi G","Zhao J","Song Y"],"additional_accession":[]},"is_claimable":false,"name":"Enhancing immunotherapy efficacy in NSCLC through the combined use of phenelzine and &lt;i&gt;Akkermansia muciniphila&lt;/i&gt; to regulate gut microbial metabolite 5-HIAA.","description":"<h4>Background</h4>Improving the efficacy of anti-programmed death 1 (PD-1) monoclonal antibody (mAb) therapy remains a major challenge for cancer immunotherapy in non-small cell lung cancer (NSCLC). Gut microbial metabolites can influence immunotherapy efficacy.<h4>Methods</h4>ELISA was used to compare the serum 5-hydroxyindoleacetic acid (5-HIAA) level in patients with NSCLC. Humanized mice were constructed to observe the effect of 5-HIAA on immunotherapy. RNA-seq and flow cytometry were used to analyze the effect of 5-HIAA on tumor-infiltrating lymphocytes. The effects of phenelzine (Phe) and <i>Akkermansia muciniphila</i> (<i>AKK</i>) on 5-HIAA synthesis, antitumor immunity and immunotherapy efficacy were analyzed. Finally, the synergistic effect of Phe combined with <i>AKK</i> on anti","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-02T20:24:05.396Z","creation":"2026-04-20T03:09:58.743Z"},"accession":"S-EPMC12421609","cross_references":{"pubmed":["40930748"],"doi":["10.1136/jitc-2025-011831"]}}