biostudies-literatureUnknown13Yu C<h4>Background</h4>Tumor-associated macrophages (TAMs) are a type of tumor-infiltrating immune cell that play a crucial role in tumor progression. However, the roles of TAMs and their regulatory mechanisms in lung adenocarcinoma (LUAD) remain poorly understood. Therefore, we aimed to develop a novel TAM-related prognostic signature to predict survival outcomes and constructed a lncRNA-miRNA-mRNA network based on these genes.<h4>Methods</h4>Transcriptomic data, clinical data, and single-cell RNA-sequencing (scRNA-seq) data were obtained from LUAD patients were obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Differentially expressed-lncRNAs (DE-lncRNAs), miRNAs (DE-miRNAs), and mRNAs (DEGs) were identified in LUAD. Differentially expressed TAM-relatePeerJe19920https://www.ebi.ac.uk/biostudies/studies/S-EPMC12422256biostudies-literatureIntegrative analyses of single-cell and bulk RNA sequencing to construct the tumor-associated macrophage-related prognostic signature in lung adenocarcinoma.PMC12422256Li HYu CChen JTian HZhuang QDeng JLuo BYe HfalseIntegrative analyses of single-cell and bulk RNA sequencing to construct the tumor-associated macrophage-related prognostic signature in lung adenocarcinoma.<h4>Background</h4>Tumor-associated macrophages (TAMs) are a type of tumor-infiltrating immune cell that play a crucial role in tumor progression. However, the roles of TAMs and their regulatory mechanisms in lung adenocarcinoma (LUAD) remain poorly understood. Therefore, we aimed to develop a novel TAM-related prognostic signature to predict survival outcomes and constructed a lncRNA-miRNA-mRNA network based on these genes.<h4>Methods</h4>Transcriptomic data, clinical data, and single-cell RNA-sequencing (scRNA-seq) data were obtained from LUAD patients were obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Differentially expressed-lncRNAs (DE-lncRNAs), miRNAs (DE-miRNAs), and mRNAs (DEGs) were identified in LUAD. Differentially expressed TAM-relate2025-01-01T00:00:00Z20252026-04-20T03:12:30.789Z2026-04-20T03:10:03.143ZS-EPMC124222564093675810.7717/peerj.19920