{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Wu X"],"pubmed_abstract":["<h4>Background</h4>The floral color variegation of cultivar 'Sahong Tao' is distinctive and possesses significant ornamental value. Currently, there are no relevant reports on how MYB transcription factors (TFs) interact with <i>LDOX</i> promoter to regulate the flower color variegation in peach.<h4>Methods</h4>In this study, we screened for proteins that interact with the <i>LDOX</i> promoter using yeast one-hybrid (Y1H) and next-generation sequencing (NGS). The NGS data were aligned with the <i>Arabidopsis</i> database (TAIR10) utilizing Python 3.10.4. PlantTFDB was employed to identify TFs, while PlantRegMap was used to predict TFs that interact with the <i>LDOX</i> promoter. The Y1H assay verified MYB1R1 interaction with <i>LDOX</i> promoter, and Y1H-AOS predicted their binding sites. "],"journal":["PeerJ"],"pagination":["e19975"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12422263"],"repository":["biostudies-literature"],"pubmed_title":["Screening of MYB1R1 interaction with &lt;i&gt;LDOX&lt;/i&gt; promoter to regulate anthocyanin biosynthesis in peaches."],"pmcid":["PMC12422263"],"pubmed_authors":["Li Y","Du T","Hu Z","Cao Z","Kang N","Zeng J","Zhuang W","Yan C","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"Screening of MYB1R1 interaction with &lt;i&gt;LDOX&lt;/i&gt; promoter to regulate anthocyanin biosynthesis in peaches.","description":"<h4>Background</h4>The floral color variegation of cultivar 'Sahong Tao' is distinctive and possesses significant ornamental value. Currently, there are no relevant reports on how MYB transcription factors (TFs) interact with <i>LDOX</i> promoter to regulate the flower color variegation in peach.<h4>Methods</h4>In this study, we screened for proteins that interact with the <i>LDOX</i> promoter using yeast one-hybrid (Y1H) and next-generation sequencing (NGS). The NGS data were aligned with the <i>Arabidopsis</i> database (TAIR10) utilizing Python 3.10.4. PlantTFDB was employed to identify TFs, while PlantRegMap was used to predict TFs that interact with the <i>LDOX</i> promoter. The Y1H assay verified MYB1R1 interaction with <i>LDOX</i> promoter, and Y1H-AOS predicted their binding sites. ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-04-25T03:29:26.227Z","creation":"2026-04-25T03:17:36.746Z"},"accession":"S-EPMC12422263","cross_references":{"pubmed":["40936769"],"doi":["10.7717/peerj.19975"]}}