biostudies-literatureUnknown13Wu X<h4>Background</h4>The floral color variegation of cultivar 'Sahong Tao' is distinctive and possesses significant ornamental value. Currently, there are no relevant reports on how MYB transcription factors (TFs) interact with <i>LDOX</i> promoter to regulate the flower color variegation in peach.<h4>Methods</h4>In this study, we screened for proteins that interact with the <i>LDOX</i> promoter using yeast one-hybrid (Y1H) and next-generation sequencing (NGS). The NGS data were aligned with the <i>Arabidopsis</i> database (TAIR10) utilizing Python 3.10.4. PlantTFDB was employed to identify TFs, while PlantRegMap was used to predict TFs that interact with the <i>LDOX</i> promoter. The Y1H assay verified MYB1R1 interaction with <i>LDOX</i> promoter, and Y1H-AOS predicted their binding sites. PeerJe19975https://www.ebi.ac.uk/biostudies/studies/S-EPMC12422263biostudies-literatureScreening of MYB1R1 interaction with &lt;i&gt;LDOX&lt;/i&gt; promoter to regulate anthocyanin biosynthesis in peaches.PMC12422263Li YDu THu ZCao ZKang NZeng JZhuang WYan CWu XfalseScreening of MYB1R1 interaction with &lt;i&gt;LDOX&lt;/i&gt; promoter to regulate anthocyanin biosynthesis in peaches.<h4>Background</h4>The floral color variegation of cultivar 'Sahong Tao' is distinctive and possesses significant ornamental value. Currently, there are no relevant reports on how MYB transcription factors (TFs) interact with <i>LDOX</i> promoter to regulate the flower color variegation in peach.<h4>Methods</h4>In this study, we screened for proteins that interact with the <i>LDOX</i> promoter using yeast one-hybrid (Y1H) and next-generation sequencing (NGS). The NGS data were aligned with the <i>Arabidopsis</i> database (TAIR10) utilizing Python 3.10.4. PlantTFDB was employed to identify TFs, while PlantRegMap was used to predict TFs that interact with the <i>LDOX</i> promoter. The Y1H assay verified MYB1R1 interaction with <i>LDOX</i> promoter, and Y1H-AOS predicted their binding sites. 2025-01-01T00:00:00Z20252026-04-25T03:29:26.227Z2026-04-25T03:17:36.746ZS-EPMC124222634093676910.7717/peerj.19975