{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Badimon A"],"funding":["NIA NIH HHS","NINDS NIH HHS"],"pubmed_abstract":["<h4>Background</h4>Polymicrobial sepsis is an infectious disease characterized by excessive inflammation and coagulation that is linked to more severe disease pathology, organ failure, and fatality. The plasma contact system is a protein cascade in the blood that can be activated by bacteria and contributes to both inflammation and coagulation.<h4>Objectives</h4>To determine if inhibiting the plasma contact system by targeting high-molecular-weight kininogen (HK) can exert a protective effect on bacteria-induced coagulation.<h4>Methods</h4>Polymicrobial cecal slurry (CS) was prepared from donor mice and used for ex vivo and in vivo experiments. CS was used in vivo to establish a murine model of polymicrobial sepsis. CS was incubated with mouse or human plasma ex vivo. Contact system activa"],"journal":["Journal of thrombosis and haemostasis : JTH"],"pagination":["S1538-7836(25)00495-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12422282"],"repository":["biostudies-literature"],"pubmed_title":["An antibody targeting high-molecular-weight kininogen blocks contact system activation in a model of polymicrobial sepsis."],"pmcid":["PMC12422282"],"funding_grant_id":["R01 AG069987","RF1 AG069987","R01 NS102721"],"pubmed_authors":["Calvano M","Wu SJ","Strickland S","Badimon A","Norris EH"],"additional_accession":[]},"is_claimable":false,"name":"An antibody targeting high-molecular-weight kininogen blocks contact system activation in a model of polymicrobial sepsis.","description":"<h4>Background</h4>Polymicrobial sepsis is an infectious disease characterized by excessive inflammation and coagulation that is linked to more severe disease pathology, organ failure, and fatality. The plasma contact system is a protein cascade in the blood that can be activated by bacteria and contributes to both inflammation and coagulation.<h4>Objectives</h4>To determine if inhibiting the plasma contact system by targeting high-molecular-weight kininogen (HK) can exert a protective effect on bacteria-induced coagulation.<h4>Methods</h4>Polymicrobial cecal slurry (CS) was prepared from donor mice and used for ex vivo and in vivo experiments. CS was used in vivo to establish a murine model of polymicrobial sepsis. CS was incubated with mouse or human plasma ex vivo. Contact system activa","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-03T05:47:25.042Z","creation":"2026-04-25T03:14:51.38Z"},"accession":"S-EPMC12422282","cross_references":{"pubmed":["40784554"],"doi":["10.1016/j.jtha.2025.07.032"]}}